Anti-TMEPA antibody produced in rabbit
SIGMA/SAB4502441 - affinity isolated antibody
Synonym: Solid tumor-associated 1 protein
Product Type: Chemical
Immunohistochemistry Anti-TMEPA: Cat. No. SAB4502441: Immunohistochemistry analysis of paraffin-embedded human prostate tissue using Anti-TMEPA antibody. Cells on the left were treated with the Anti-TMEPA antibody. Cells on the right (negative control) were treated with both Anti-TMEPA antibody and the synthesized immunogen peptide.
Immunofluorescence Anti-TMEPA: Cat. No. SAB4502441: Immunofluorescence of HuvEc cells using Anti-TMEPA antibody. Cells on the left were treated with the Anti-TMEPA antibody. Cells on the right (negative control) were treated with both Anti-TMEPA antibody and the synthesized immunogen peptide.
Western Blotting Anti-TMEPA: Cat. No. SAB4502441: Immunoblotting analysis of extracts from HT-29 cells, using Anti-TMEPA antibody. The lane on the left was treated with the Anti-TMEPA antibody. The lane on the right (negative control) was treated with both Anti-TMEPA antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 31 kDa |
| NCBI accession no. | Q969W9   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:20000 |
| immunofluorescence: 1:100-1:500 | |
| immunohistochemistry: 1:50-1:100 | |
| western blot: 1:500-1:1000 |
| Biochem/physiol Actions: | Transmembrane prostate androgen-induced RNA (TMEPAI)/ prostate transmembrane protein androgen induced 1 (PMEPA1) plays a vital role in maintaining prostate homeostasis. It is also implicated in p53-mediated apoptosis and cell growth inhibition. TMEPAI negatively regulate transforming growth factor- β (TGF-β) signaling in mammalian cells and Xenopus embryos. Upregulated expression of PMEPA1 stimulates cell migration and conserves the mesenchymal-like morphology of breast cancer cells. Deletion of the gene leads to prostate cancer. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Anti-TMEPA Antibody detects endogenous levels of total TMEPA protein. |
| General description: | The transmembrane prostate androgen-induced RNA (TMEPAI), also known as prostate transmembrane protein androgen induced 1 (PMEPA1) gene, spans 55-60 kb on human chromosome 20q13.31-q13.33. The encoded protein is mainly expressed in prostate gland and is regulated by androgen receptor (AR). TMEPAI is a type Ib transmembrane protein characterized with two PY motifs involved in binding HECT(homologous to the E6AP carboxyl terminus)-type E3 ubiquitin ligases. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human TMEPA. Immunogen Range: 101-150 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |