Anti-PTHR1 antibody produced in rabbit
SIGMA/SAB4502493 - affinity isolated antibody
Synonym: PTH1 Receptor; PTH1R; PTHR; Parathyroid hormone 1 receptor
MDL Number: MFCD02263454
Product Type: Chemical
Immunohistochemistry Anti-PTHR1: Cat. No. SAB4502493: Immunohistochemistry analysis of paraffin-embedded human brain tissue using Anti-PTHR1 antibody. Cells on the left were treated with the Anti-PTHR1 antibody. Cells on the right (negative control) were treated with both Anti-PTHR1 antibody and the synthesized immunogen peptide.
Immunofluorescence Anti-PTHR1: Cat. No. SAB4502493: Immunofluorescence of MCF7 cells using Anti-PTHR1 antibody. Cells on the left were treated with the Anti-PTHR1 antibody. Cells on the right (negative control) were treated with both Anti-PTHR1 antibody and the synthesized immunogen peptide.
Western Blotting Anti-PTHR1: Cat. No. SAB4502493: Immunoblotting analysis of extracts from Jurkat cells, using Anti-PTHR1 antibody. The lane on the left was treated with the Anti-PTHR1 antibody. The lane on the right (negative control) was treated with both Anti-PTHR1 antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 52 kDa |
| NCBI accession no. | Q03431   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | rat, mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:10000 |
| immunofluorescence: 1:100-1:500 | |
| immunohistochemistry: 1:50-1:100 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | Q03431 |
| Biochem/physiol Actions: | Heterozygous mutation of PTHR1 (parathyroid hormone 1 receptor) gene is known to cause primary failure of tooth eruption in human, with partial eruption of posterior teeth and reduced alveolar growth process. PTHR1 is associated with bone remodeling (bone formation and resorption, anabolic and catabolic responses respectively) and calcium homeostasis. Blomstrand chondrodysplasia results due to complete loss of PTHR1 function, and is considered lethal. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Anti-PTHR1 antibody detects endogenous levels of total PTHR1 protein. |
| General description: | PTHR1 (parathyroid hormone 1 receptor) gene is mapped to human chromosome 3p21.31. The gene encodes for a precursor (secretin-like class II G protein-coupled receptor) that matures to a receptor containing 593 amino acids. This receptor contains an extracellular loop with a PTH binding site and an intracellular signaling domain. It also includes seven transmembrane and helical segments. The gene is predominantly expressed in osteoblasts and renal tubular cells. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human PTHR1. Immunogen Range: 145-194 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |