Anti-CD45 antibody produced in rabbit
SIGMA/SAB4502541 - affinity isolated antibody
Synonym: L-CA; LY-5; Leukocyte common antigen precursor; Leukocyte common antigen variant 4 precursor; Lymphocyte common antigen Ly-5
Product Type: Chemical
Immunohistochemistry Anti-CD45: Cat. No. SAB4502541: Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-CD45 antibody. Cells on the left were treated with the Anti-CD45 antibody. Cells on the right (negative control) were treated with both Anti-CD45 antibody and the synthesized immunogen peptide.
Immunofluorescence Anti-CD45: Cat. No. SAB4502541: Immunofluorescence of HuvEc cells using Anti-CD45 antibody. Cells on the left were treated with the Anti-CD45 antibody. Cells on the right (negative control) were treated with both Anti-CD45 antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 147 kDa |
| NCBI accession no. | P08575   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | rat, mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:1000 |
| immunofluorescence: 1:100-1:500 | |
| immunohistochemistry: 1:50-1:100 | |
| UniProt accession no. | P08575 |
| Application: | Anti-CD45 antibody produced in rabbit has been used in: • immunohistochemistry staining • immunofluorescence staining • immunostaining |
| Biochem/physiol Actions: | The Cluster of differentiation 45 (CD45) facilitates the regulation of lymphocyte signaling and SRC family kinase Lck. This protein participates in the polymorphism in several mammalian species. |
| Biochem/physiol Actions: | The protein encoded by CD45 (cluster of differentiation 45) is a prototypic receptor and exhibits phosphatase activity. CD45 is found to control T-cell mediated BCR-signaling. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Anti-CD45 antibody detects endogenous levels of total CD45 protein. |
| General description: | The CD45 (cluster of differentiation 45) gene is mapped to human chromosome 1q31.3-q32.1. It codes for a type I transmembrane protein and is expressed in nucleated hematopoietic cells along within its precursors. The protein consists of a varying extracellular domain and a highly conserved intracellular domain. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human CD45. Immunogen Range: 981-1030 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |