Anti-BAX antibody produced in rabbit
SIGMA/SAB4502546 - affinity isolated antibody
Synonym: Apoptosis regulator BAX; BAX; BCL2L4; Bcl-2-like protein 4; Bcl2-L-4
Product Type: Chemical
Immunofluorescence Anti-BAX: Cat. No. SAB4502546: Immunofluorescence of HuvEc cells using Anti-BAX antibody. Cells on the left were treated with the Anti-BAX antibody. Cells on the right (negative control) were treated with both Anti-BAX antibody and the synthesized immunogen peptide.
Western Blotting Anti-BAX: Cat. No. SAB4502546: Immunoblotting analysis of extracts from HepG2 cells, using Anti-BAX antibody. The lane on the left was treated with the Anti-BAX antibody. The lane on the right (negative control) was treated with both Anti-BAX antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 21 kDa |
| NCBI accession no. | Q07812   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | rat, human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:1000 |
| immunofluorescence: 1:100-1:500 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | Q07812 |
| Application: | Anti-BAX, C-Terminal antibody produced in rabbit has been used in western blotting analysis. |
| Biochem/physiol Actions: | BAX (BCL2 (B-cell lymphoma 2) associated X, apoptosis regulator) is predominantly associated with cellular homeostasis, cell cycle and pathogenesis of cell death particularly in cancer cells. In response to an apoptotic stimulus, Bax brings about mitochondrial dysfunction and apoptosis. Oligomerization of bax promotes mitochondrial outer membrane permeabilization. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Anti-BAX antibody detects endogenous levels of total BAX protein. |
| General description: | The BAX (BCL2 (B-cell lymphoma 2) associated X, apoptosis regulator) gene is mapped to human chromosome 19q13.33. It codes for α-helical globular protein. The protein contains eight amphipathic helices around a hydrophobic helix. Bax is known to be mostly localized in the cytoplasm. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human Bax. Immunogen Range: 133-182 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |