Anti-RAC1 antibody produced in rabbit
SIGMA/SAB4502560 - affinity isolated antibody
Synonym: Cell migration-inducing gene 5 protein; RAC1; Ras-like protein TC25; Ras-related C3 botulinum toxin substrate 1; p21-Rac1
MDL Number: MFCD01322594
Product Type: Chemical
Immunohistochemistry Anti-RAC1: Cat. No. SAB4502560: Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Anti-RAC1 antibody. Cells on the left were treated with the Anti-RAC1 antibody. Cells on the right (negative control) were treated with both Anti-RAC1 antibody and the synthesized immunogen peptide.
Western Blotting Anti-RAC1: Cat. No. SAB4502560: Immunoblotting analysis of extracts from NIH-3T3 cells, treated with EGF 200ng/ml 30′, using Anti-RAC1 antibody. The lane on the left was treated with the Anti-RAC1 antibody. The lane on the right (negative control) was treated with both Anti-RAC1 antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 21 kDa |
| NCBI accession no. | P63000   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | rat, human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:20000 |
| immunohistochemistry: 1:50-1:100 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P60763 |
| Biochem/physiol Actions: | Ras-related C3 botulinum toxin substrate 1 (RAC1), expressed in decidua, modulates uterine secretions that helps in maternal-fetal communication crucial for placental development and establishment of pregnancy. RAC1 regulates the secretory activity of human endometrial stromal cells during decidualization. Members of rho family of small GTPases, including RAC1, is associated with signal transduction pathways that regulate proliferation, adhesion, and migration of cells during embryonic development and invasiveness of tumor cells. RAC1 acts as a regulator of nuclear factor-κB (NF-κB) signaling pathway, and it is also associated with inflammation.Hindrance of RAC1 expression in lens epithelial cells can be a potential method for the treatment of cataract. Elevated expression of RAC1 promotes the proliferation, migration, and invasion of lens epithelial cells, and regulates the epithelial-mesenchymal transition (EMT) process, thus, RAC1 is associated with cataract. RAC1 functions as an oncogene in tumor cells. Deletion of RAC1 retards cell migration and wound healing of epidermal keratinocytes. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | The gene ras-related C3 botulinum toxin substrate 1 (RAC1), with 7 exons spanning 29 kb, is mapped to human chromosome 7p22. The encoded protein belongs to the Rho family of small GTPases. RAC1 is a maternal factor expressed in decidual cells. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human Rac1/CDC42. Immunogen Range: 38-87 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |