Anti-RAD21 antibody produced in rabbit
SIGMA/SAB4502562 - affinity isolated antibody
Synonym: Double-strand-break repair protein rad21 homolog; Hhr21; NXP-1; Nuclear matrix protein 1; SCC1 homolog
Product Type: Chemical
Western Blotting Anti-RAD21: Cat. No. SAB4502562: Immunoblotting analysis of extracts from Jurkat cells, using Anti-RAD21 antibody. The lane on the left was treated with the Anti-RAD21 antibody. The lane on the right (negative control) was treated with both Anti-RAD21 antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 71 kDa |
| NCBI accession no. | O60216   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:1000 |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | O60216 |
| Application: | Anti-RAD21, C-Terminal antibody produced in rabbit is suitable for indirect ELISA and western blot applications. |
| Biochem/physiol Actions: | RAD21 (RAD21 homolog, S. pombe) plays a crucial role in normal cell division. It acts as a main component of the cohesin complex for the proper chromosomal arrangement during cell division. The protein possesses two mitotic cleavage sites for the attachment of separase. It has been shown experimentally that at the beginning of anaphase stage, a caspase-like Esp1/separase attaches to the cleavage site of RAD21 and initiate sister chromatid separation, which is an essential step for the completion of cytokinesis. During double strand DNA damage repair, it helps the two DNA strands to adhere with each other. Mutation in RAD21 causes a congenital phenotype, cohesinopathy in humans characterized with growth retardation and minor skeletal anomalies. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Anti-RAD21 Antibody detects endogenous levels of total RAD21 protein. |
| General description: | RAD21 (RAD21 homolog, S. pombe) is a novel nuclear protein located on chromosome 8q24. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human RAD21. Immunogen Range: 521-570 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |