Anti-C-RAF antibody produced in rabbit
SIGMA/SAB4502573 - affinity isolated antibody
Synonym: C-RAF; C-Raf; CRAF; Protein kinase raf 1; kinase Raf1
Product Type: Chemical
Immunohistochemistry Anti-C-RAF: Cat. No. SAB4502573: Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Anti-C-RAF antibody. Cells on the left were treated with the Anti-C-RAF antibody. Cells on the right (negative control) were treated with both Anti-C-RAF antibody and the synthesized immunogen peptide.
Western Blotting Anti-C-RAF: Cat. No. SAB4502573: Immunoblotting analysis of extracts from 293 cells, treated with PMA 125ng/ml 30′ , using Anti-C-RAF antibody. The lane on the left was treated with the Anti-C-RAF antibody. The lane on the right (negative control) was treated with both Anti-C-RAF antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 73 kDa |
| NCBI accession no. | P04049   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | mouse, human, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:40000 |
| immunohistochemistry: 1:50-1:100 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P04049 |
| Biochem/physiol Actions: | Raf-1 proto-oncogene, serine/threonine kinase (RAF1) or C-RAF is a crucial component of the Raf/mitogen-activated extracellular signal-regulated kinase (MEK)/extracellular regulated protein kinase (ERK) signaling cascade. It functions as an activator of MEK-1 and -2. The protein is upregulated in many cancers. Mutations in the gene encoding RAF1 have been linked to Noonan syndrome. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Anti-C-RAF Antibody detects endogenous levels of total C-RAF protein. Raf-1 proto-oncogene, serine/threonine kinase (RAF1) or C-RAF is an anti-apoptotic protein. The gene encoding it is localized on human chromosome 3p25.2. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human C-RAF. Immunogen Range: 271-320 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |