Anti-Retinoblastoma antibody produced in rabbit
SIGMA/SAB4502585 - affinity isolated antibody
Synonym: P105-RB; PP105; PP110; RB-1; RB1
MDL Number: MFCD00282455
Product Type: Chemical
Immunohistochemistry Anti-Retinoblastoma: Cat. No. SAB4502585: Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using Anti-Retinoblastoma antibody. Cells on the left were treated with the Anti-Retinoblastoma antibody. Cells on the right (negative control) were treated with both Anti-Retinoblastoma antibody and the synthesized immunogen peptide.
Immunofluorescence Anti-Retinoblastoma: Cat. No. SAB4502585: Immunofluorescence of HeLa cells using Anti-Retinoblastoma antibody. Cells on the left were treated with the Anti-Retinoblastoma antibody. Cells on the right (negative control) were treated with both Anti-Retinoblastoma antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 106 kDa |
| NCBI accession no. | P06400   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | rat, human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:5000 |
| immunofluorescence: 1:100-1:500 | |
| immunohistochemistry: 1:50-1:100 | |
| UniProt accession no. | P06400 |
| Biochem/physiol Actions: | Retinoblastoma-associated protein (RB1) modulates the cell cycle. It acts as a repressor of transcription mediated by E2 factor (E2F) and influences transcriptional silencing. RB1 modulates mitochondrial activities and has a role in the organisation of heterochromatin. Mutations in the gene encoding this protein have been associated with childhood retinoblastoma. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | The gene encoding Retinoblastoma-associated protein (RB1) is a tumor suppressor gene localized on human chromosome 13q14.2. Anti-Retinoblastoma Antibody detects endogenous levels of total Retinoblastoma protein. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human Retinoblastoma. Immunogen Range: 211-260 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |