Anti-Retinoblastoma, C-Terminal antibody produced in rabbit
SIGMA/SAB4502589 - affinity isolated antibody
Synonym: P105-RB; PP105; PP110; RB-1; RB1
MDL Number: MFCD00282455
Product Type: Chemical
Immunohistochemistry Anti-Retinoblastoma: Cat. No. SAB4502589: Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Anti-Retinoblastoma antibody. Cells on the left were treated with the Anti-Retinoblastoma antibody. Cells on the right (negative control) were treated with both Anti-Retinoblastoma antibody and the synthesized immunogen peptide.
Western Blotting Anti-Retinoblastoma: Cat. No. SAB4502589: Immunoblotting analysis of extracts from MOLT cells, using Anti-Retinoblastoma antibody. The lane on the left was treated with the Anti-Retinoblastoma antibody. The lane on the right (negative control) was treated with both Anti-Retinoblastoma antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 106 kDa |
| NCBI accession no. | P06400   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human, rat, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:20000 |
| immunohistochemistry: 1:50-1:100 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P06400 |
| Biochem/physiol Actions: | Retinoblastoma-associated protein (RB1) modulates the cell cycle. It acts as a repressor of transcription mediated by E2 factor (E2F) and influences transcriptional silencing. RB1 modulates mitochondrial activities and has a role in the organisation of heterochromatin. Mutations in the gene encoding this protein have been associated with childhood retinoblastoma. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | The gene encoding Retinoblastoma-associated protein (RB1) is a tumor suppressor gene localized on human chromosome 13q14.2. Anti-Retinoblastoma Antibody detects endogenous levels of total Retinoblastoma protein. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human Retinoblastoma. Immunogen Range: 781-830 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |