Anti-NF-κB p65 (Acetyl-Lys310) antibody produced in rabbit
SIGMA/SAB4502616 - affinity isolated antibody
Synonym: NFKB3; RELA; TF65; nuclear factor NF-κ-B p65 subunit; p65
Product Type: Chemical
Immunohistochemistry Anti-NF-κB p65 (Acetyl-Lys310): Cat. No. SAB4502616: Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using Anti-NF-κB p65 (Acetyl-Lys310) antibody. Cells on the left were treated with the Anti-NF-κB p65 (Acetyl-Lys310) antibody. Cells on the right (negative control) were treated with both Anti-NF-κB p65 (Acetyl-Lys310) antibody and the synthesized immunogen peptide.
Immunofluorescence Anti-NF-κB p65 (Acetyl-Lys310): Cat. No. SAB4502616: Immunofluorescence of HeLa cells using Anti-NF-κB p65 (Acetyl-Lys310) antibody. Cells on the left were treated with the Anti-NF-κB p65 (Acetyl-Lys310) antibody. Cells on the right (negative control) were treated with both Anti-NF-κB p65 (Acetyl-Lys310) antibody and the synthesized immunogen peptide.
Western Blotting Anti-NF-κB p65 (Acetyl-Lys310): Cat. No. SAB4502616: Immunoblotting analysis of extracts from HeLa cells, treated with TSA 40nM 24h, using Anti-NF-κB p65 (Acetyl-Lys310) antibody. The lane on the left was treated with the Anti-NF-κB p65 (Acetyl-Lys310) antibody. The lane on the right (negative control) was treated with both Anti-NF-κB p65 (Acetyl-Lys310) antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 60 kDa |
| NCBI accession no. | Q04206   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | mouse, human |
| storage temp. | −20°C |
| target post-translational modification | acetylation (Lys310) |
| technique(s) | ELISA: 1:40000 |
| immunofluorescence: 1:100-1:500 | |
| immunohistochemistry: 1:50-1:100 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | Q04206 |
| Application: | Anti-NF-κB p65 (Acetyl-Lys310) antibody has been used in western blotting. |
| Biochem/physiol Actions: | RELA (v-rel avian reticuloendotheliosis viral oncogene homolog A) participates in nuclear factor κ-B (NFKB) signaling pathway. It also controls inflammation. Alterations in the gene results in schizophrenia and startle reactions. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Anti-NF-κB p65 (Acetyl-Lys310) Antibody detects endogenous levels of total NF-κB p65 (Acetyl-Lys310) protein. RELA (v-rel avian reticuloendotheliosis viral oncogene homolog A) codes for the important constituent of the NF-κB (nuclear factor κ-B) complex. It is located on human chromosome 11q13.1. It is also called as nuclear factor κB subunit 3 (NFKB3). |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human NF-kappaB p65 around the acetylated site of Lys310. Immunogen Range: 275-324 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |