Anti-DNA Polymerase ζ, N-Terminal antibody produced in rabbit
SIGMA/SAB4502626 - affinity isolated antibody
Synonym: DNA polymerase ζ catalytic subunit; DPOLZ; DPOZ; POLZ; REV3L
Product Type: Chemical
Immunohistochemistry Anti-DNA Polymerase ζ: Cat. No. SAB4502626: Immunohistochemistry analysis of paraffin-embedded human brain tissue using Anti-DNA Polymerase ζ antibody. Cells on the left were treated with the Anti-DNA Polymerase ζ antibody. Cells on the right (negative control) were treated with both Anti-DNA Polymerase ζ antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 352 kDa |
| NCBI accession no. | O60673   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:20000 |
| immunohistochemistry: 1:50-1:100 | |
| UniProt accession no. | O60673 |
| Biochem/physiol Actions: | REV3L (reversionless 3-like) is predominantly involved in DNA damage response, induced by ultraviolet radiation and chemical damaging agents such as cisplatin. The encoded protein DNA Polymerase ζ catalyzes the translesion synthesis (TLS) with less stringent and lower processivity. Loss of gene function is embryonically lethal. Upregulation of REV3L results in spontaneous mutations. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
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| General description: | Anti-DNA Polymerase ζ antibody detects endogenous levels of total DNA polymerase ζ protein. |
| General description: | The REV3L (reversionless 3-like) gene is mapped to human chromosome 6q21. It codes for a ~350 kDa protein DNA polymerase ζ which localizes to mitochondria. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human DNA Polymerase zeta. Immunogen Range: 231-280 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |