Anti-Actin-pan antibody produced in rabbit
SIGMA/SAB4502631 - affinity isolated antibody
Synonym: ACTA; ACTA1; ACTS; Actin α skeletal muscle
Product Type: Chemical
Immunohistochemistry Anti-Actin-pan: Cat. No. SAB4502631: Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue using Anti-Actin-pan antibody. Cells on the left were treated with the Anti-Actin-pan antibody. Cells on the right (negative control) were treated with both Anti-Actin-pan antibody and the synthesized immunogen peptide.
Immunofluorescence Anti-Actin-pan: Cat. No. SAB4502631: Immunofluorescence of HuvEc cells using Anti-Actin-pan antibody. Cells on the left were treated with the Anti-Actin-pan antibody. Cells on the right (negative control) were treated with both Anti-Actin-pan antibody and the synthesized immunogen peptide.
Western Blotting Anti-Actin-pan: Cat. No. SAB4502631: Immunoblotting analysis of extracts from mouse brain cells, using Anti-Actin-pan antibody. The lane on the left was treated with the Anti-Actin-pan antibody. The lane on the right (negative control) was treated with both Anti-Actin-pan antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 41 kDa |
| NCBI accession no. | P68032   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | rat, mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:10000 |
| immunofluorescence: 1:100-1:500 | |
| immunohistochemistry: 1:50-1:100 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P68032 |
| Biochem/physiol Actions: | Mutations in ACTA1 (actin, α1) is known to cause thin filament myopathy, such as nemaline myopathy, congenital myopathy, actin myopathy, intranuclear rod myopathy, cap myopathy and core myopathy. Upregulation of the gene is observed in orthotopic liver transplantation without any earlier evidence of autoimmune liver disease. The encoded protein actin forms a network of filaments and is responsible for the shape of the cell. It provides cellular strength and promotes active movement of the cell. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Anti-Actin-pan antibody detects endogenous levels of total actin-pan protein. |
| General description: | The ACTA1 (actin, α1) gene is mapped to human chromosome 1q42.13. The gene codes for actin 1, a thin filament component of skeletal muscle. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human Actin. Immunogen Range: 21-70 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |