Anti-RYR2 antibody produced in rabbit
SIGMA/SAB4502707 - affinity isolated antibody
Synonym: RYR-2; cardiac muscle ryanodine receptor-calcium release channel; cardiac muscle-type ryanodine receptor; ryanodine receptor 2
Product Type: Chemical
Immunohistochemistry Anti-RYR2: Cat. No. SAB4502707: Immunohistochemistry analysis of paraffin-embedded human brain tissue using Anti-RYR2 antibody. Cells on the left were treated with the Anti-RYR2 antibody. Cells on the right (negative control) were treated with both Anti-RYR2 antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 564 kDa |
| NCBI accession no. | Q92736   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human, mouse, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:1000 |
| immunohistochemistry: 1:50-1:100 | |
| UniProt accession no. | Q92736 |
| Application: | Anti-RYR2 antibody produced in rabbit is suitable for immunohistochemistry and indirect ELISA. |
| Biochem/physiol Actions: | RYR2 (ryanodine receptor 2) provides guidance to the release and transport of Ca2+ from sarcoplasmic reticulum (SR) to the cytoplasm during cardiac muscle excitation-contraction (EC) coupling. Protein kinase A (PKA) phosphorylated RYR2 separates (FKBP12.6) to regulate the channel open probability (Po). During the process, a small portion of Ca2+ enter into the cell through the L-type Ca2+ channel, which further activates the RyR2 channel upon membrane depolarization. The activated RyR2 channel releases a large amount of Ca2+ from the SR and subsequent muscle contraction. Missense mutations in this gene cause Kazakh idiopathic ventricular tachycardia and arrhythmogenic right ventricular dysplasia. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Anti-RYR2 Antibody detects endogenous levels of total RYR2 protein. |
| General description: | RYR2 (ryanodine receptor 2) is an intracellular Ca2+ release channel present on the sarcoplasmic reticulum (SR). It forms tetramer with four types 2 RyR polypeptides (RyR2) and four FK506 binding proteins (FKBP12.6). |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human RyR2. Immunogen Range: 2774-2823 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |