Anti-Syndecan4 antibody produced in rabbit
SIGMA/SAB4502721 - affinity isolated antibody
Synonym: Amphiglycan; Ryudocan core protein; SDC4; SYND4; Syndecan-4 precursor
Product Type: Chemical
Immunohistochemistry Anti-Syndecan4: Cat. No. SAB4502721: Immunohistochemistry analysis of paraffin-embedded human brain tissue using Anti-Syndecan4 antibody. Cells on the left were treated with the Anti-Syndecan4 antibody. Cells on the right (negative control) were treated with both Anti-Syndecan4 antibody and the synthesized immunogen peptide.
Immunofluorescence Anti-Syndecan4: Cat. No. SAB4502721: Immunofluorescence of HeLa cells using Anti-Syndecan4 antibody. Cells on the left were treated with the Anti-Syndecan4 antibody. Cells on the right (negative control) were treated with both Anti-Syndecan4 antibody and the synthesized immunogen peptide.
Western Blotting Anti-Syndecan4: Cat. No. SAB4502721: Immunoblotting analysis of extracts from HepG2 cells, using Anti-Syndecan4 antibody. The lane on the left was treated with the Anti-Syndecan4 antibody. The lane on the right (negative control) was treated with both Anti-Syndecan4 antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 21 kDa |
| NCBI accession no. | P31431   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | rat, human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:40000 |
| immunofluorescence: 1:100-1:500 | |
| immunohistochemistry: 1:50-1:100 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P31431 |
| Biochem/physiol Actions: | Syndecan-4 (SDC-4) is associated with bone development and osteogenesis by regulating fibroblast growth factor-2 (FGF2). SDC-4 gene expression promotes cell division, migration and adhesion. It is essential for wound healing and takes part in inflammation and fibrosis. An abnormal SDC-4 expression is observed in response to stress and metastatic tumor. Growth factors such as FGF2, TNF-α (tumor necrosis factor α) and TGF-β (transforming growth factor β) also tend to alter SDC-4 expression. It is implicated as a biomarker in cardiovascular disease. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | The SDC-4 (syndecan-4) gene encodes a membrane-spanning proteoglycan which can bear heparan sulfate and chondroitin sulfate chains. Syndecan-4 is predominantly expressed on smooth muscle cells, epithelial cells and cardiomyocytes. The SDC-4 gene is mapped to human chromosome 20q13.12. Anti-Syndecan4 antibody detects endogenous levels of total syndecan-4 protein. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human Syndecan4. Immunogen Range: 145-194 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |