Anti-SNAP25 antibody produced in rabbit
SIGMA/SAB4502826 - affinity isolated antibody
Synonym: SN25; SNAP; SNP25; SUP; Super protein
Product Type: Chemical
Immunohistochemistry Anti-SNAP25: Cat. No. SAB4502826: Immunohistochemistry analysis of paraffin-embedded human brain tissue using Anti-SNAP25 antibody. Cells on the left were treated with the Anti-SNAP25 antibody. Cells on the right (negative control) were treated with both Anti-SNAP25 antibody and the synthesized immunogen peptide.
Western Blotting Anti-SNAP25: Cat. No. SAB4502826: Immunoblotting analysis of extracts from Raw264.7 cells, treated with EGF 200ng/ml 30′, using Anti-SNAP25 antibody. The lane on the left was treated with the Anti-SNAP25 antibody. The lane on the right (negative control) was treated with both Anti-SNAP25 antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 23 kDa |
| NCBI accession no. | P60880   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | mouse, human, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:20000 |
| immunohistochemistry: 1:50-1:100 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P60880 |
| Biochem/physiol Actions: | SNAP25 (synaptosome associated protein 25) protein is involved in extracellular trafficking and mediates the process of exocytosis and endocytosis by promoting fusion of vesicles with the plasma membrane. SNAP25 is needed for rapid excitatory and inhibitory neurotransmission of neurons producing glutamate and GABA (gamma aminobutyric acid). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Anti-SNAP25 antibody detects endogenous levels of total SNAP25 protein. |
| General description: | The SNAP25 (synaptosome associated protein 25) gene is mapped to human chromosome 20p12.2. The encoded protein belongs to the family of SNARE (small ILF3/NF90-associated RNA E) complex. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human SNAP25. Immunogen Range: 151-200 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |