Anti-SOX9 antibody produced in rabbit
SIGMA/SAB4502834 - affinity isolated antibody
Synonym: SRY sex determining region Y-box 9 protein; SRY-box containing gene 9; Sox-9; transcription factor SOX-9
MDL Number: MFCD03792047
Product Type: Chemical
Immunohistochemistry Anti-SOX9: Cat. No. SAB4502834: Immunohistochemistry analysis of paraffin-embedded human brain tissue using Anti-SOX9 antibody. Cells on the left were treated with the Anti-SOX9 antibody. Cells on the right (negative control) were treated with both Anti-SOX9 antibody and the synthesized immunogen peptide.
Immunofluorescence Anti-SOX9: Cat. No. SAB4502834: Immunofluorescence of A549 cells using Anti-SOX9 antibody. Cells on the left were treated with the Anti-SOX9 antibody. Cells on the right (negative control) were treated with both Anti-SOX9 antibody and the synthesized immunogen peptide.
Western Blotting Anti-SOX9: Cat. No. SAB4502834: Immunoblotting analysis of extracts from 293 cells, treated with PBS 60′, using Anti-SOX9 antibody. The lane on the left was treated with the Anti-SOX9 antibody. The lane on the right (negative control) was treated with both Anti-SOX9 antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 56 kDa |
| NCBI accession no. | P48436   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:5000 |
| immunofluorescence: 1:100-1:500 | |
| immunohistochemistry: 1:50-1:100 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P48436 |
| Biochem/physiol Actions: | The protein encoded by SOX9 (SRY (sex determining region Y)-box 9) gene is involved in the regulation of developmental processes such as chondrogenesis, gliogenesis, epithelial hair follicle stem cell specification, gonadogenesis, normal development of septum, lung, cardiac valve and pancreas. Abnormal regulation of SOX9 promotes tumorigenesis in different tissues. SOX9 induces the process of metastasis in cancer cells. Overexpression of the gene is observed in prostate, breast, and colorectal cancers. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Anti-SOX9 antibody detects endogenous levels of total SOX9 protein. |
| General description: | The SOX9 (SRY (sex determining region Y)-box 9) gene is located on human chromosome 17q24.3. SOX9 codes for a DNA-binding protein that belongs to the class of high-mobility-group box. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human SOX9. Immunogen Range: 147-196 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |