Anti-SP1 antibody produced in rabbit
SIGMA/SAB4502837 - affinity isolated antibody
Synonym: TSFP1; Transcription factor SP1; transcription factor Sp1
Product Type: Chemical
Immunohistochemistry Anti-SP1: Cat. No. SAB4502837: Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-SP1 antibody. Cells on the left were treated with the Anti-SP1 antibody. Cells on the right (negative control) were treated with both Anti-SP1 antibody and the synthesized immunogen peptide.
Immunofluorescence Anti-SP1: Cat. No. SAB4502837: Immunofluorescence of HepG2 cells using Anti-SP1 antibody. Cells on the left were treated with the Anti-SP1 antibody. Cells on the right (negative control) were treated with both Anti-SP1 antibody and the synthesized immunogen peptide.
Western Blotting Anti-SP1: Cat. No. SAB4502837: Immunoblotting analysis of extracts from HT-29 cells, using Anti-SP1 antibody. The lane on the left was treated with the Anti-SP1 antibody. The lane on the right (negative control) was treated with both Anti-SP1 antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 80 kDa |
| NCBI accession no. | P08047   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | rat, human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:20000 |
| immunofluorescence: 1:100-1:500 | |
| immunohistochemistry: 1:50-1:100 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P08047 |
| Biochem/physiol Actions: | Sp1 protein was shown to recognize and bind selectively to a GC-rich consensus sequence (GC-box: GGGCGG or CACCC) that presents in the promoter of several important cellular genes, including Simian vacuolating virus 40 (SV40) early, human immunodeficiency virus-1 (HIV-1), platelet-derived growth factor subunit B (PDGF-B), Myc, c-Src etc. In addition to transcription, Sp1 function has been linked to cell growth, cancer and Huntington disease. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Sp1 protein was the first transcription factor to be cloned and characterized. It was first detected in HeLa cells on the basis of its ability to activate the SV40 early promoter transcription. Analysis of structure and function has revealed that Sp1 can be separated into discrete functional domains. The DNA-binding domain consists of three zinc fingers that specifically bind to the GC-box element. Anti-SP1 Antibody detects endogenous levels of total SP1 protein. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human SP1. Immunogen Range: 706-755 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |