Anti-AURA antibody produced in rabbit
SIGMA/SAB4502887 - affinity isolated antibody
Synonym: AIK; ARK1; Aurora-A; Aurora-family kinase 1; Aurora-related kinase 1
Product Type: Chemical
Western Blotting Anti-AURA: Cat. No. SAB4502887: Immunoblotting analysis of extracts from COS7 cells, using Anti-AURA antibody. The lane on the left was treated with the Anti-AURA antibody. The lane on the right (negative control) was treated with both Anti-AURA antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 45 kDa |
| NCBI accession no. | O14965   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | mouse, rat, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:5000 |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | O14965 |
| Biochem/physiol Actions: | AURA/AURKA (aurora kinase A) modulates cell cycle checkpoints and plays important roles in centrosome division, cytoskeleton and cytokinesis. It sustains the integrity of chromosome after DNA damage. The catalytic domain of AURKA possesses a serine/threonine kinase activity role. It is overexpressed in several malignancies like invasive carcinomas of ovary, head, neck and breast. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | AURA/AURKA (aurora kinase A) is mapped on to human chromosome 20q13.2. It is also termed as STK15 (serine/threonine kinase 15). This gene codes for a protein of 403 amino acids with regulatory and catalytic domains. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human AurA. Immunogen Range: 311-360 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |