Anti-Claudin 5, C-Terminal antibody produced in rabbit
SIGMA/SAB4502981 - affinity isolated antibody
Synonym: CLD5; CLDN5; TMDVCF; Transmembrane protein deleted in VCFS
MDL Number: MFCD03454913
Product Type: Chemical
Immunohistochemistry Anti-Claudin 5: Cat. No. SAB4502981: Immunohistochemistry analysis of paraffin-embedded human brain tissue using Anti-Claudin 5 antibody. Cells on the left were treated with the Anti-Claudin 5 antibody. Cells on the right (negative control) were treated with both Anti-Claudin 5 antibody and the synthesized immunogen peptide.
Western Blotting Anti-Claudin 5: Cat. No. SAB4502981: Immunoblotting analysis of extracts from A549 cells, using Anti-Claudin 5 antibody. The lane on the left was treated with the Anti-Claudin 5 antibody. The lane on the right (negative control) was treated with both Anti-Claudin 5 antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 23 kDa |
| NCBI accession no. | O00501   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | mouse, human, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:40000 |
| immunohistochemistry: 1:50-1:100 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | O00501 |
| Application: | Anti-Claudin 5, C-Terminal antibody produced in rabbit has been used for intravascular perfusion labelling of vascular endothelial- cadherin and Immunofluorescence. |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Immunocytochemistry (1 paper) |
| Biochem/physiol Actions: | The CLDN5 (claudins) gene encodes a component of tight junction and serves as a selective barrier for solutes and water. Upregulation of CLDN5 was observed in migraine. The expression of CLDN5 is reduced in lung squamous carcinoma. CLDN5 is known to increase p21 and decrease cyclin D1, leading to the retardation of cell cycle G1 to S transition. Cldn5 maintains homeostasis of the central nervous system, being a part of blood-brain barrier. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
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| General description: | Anti-Claudin 5 Antibody detects endogenous levels of total Claudin 5 protein. |
| General description: | The CLDN5 (claudins) gene is mapped to human chromosome 22q11.21 and codes for a transmembrane protein, which is a component of tight junction. The protein is a part of blood-brain barrier. The encoded protein is 303 - 218 amino acids long. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human Claudin 5. Immunogen Range: 169-218 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |