Anti-TNFA antibody produced in rabbit
SIGMA/SAB4502982 - affinity isolated antibody
Synonym: Cachectin; TNF-α; TNF-a; Tumor necrosis factor; Tumor necrosis factor ligand superfamily member 2
MDL Number: MFCD00162116
Product Type: Chemical
Immunohistochemistry Anti-TNFA: Cat. No. SAB4502982: Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-TNFA antibody. Cells on the left were treated with the Anti-TNFA antibody. Cells on the right (negative control) were treated with both Anti-TNFA antibody and the synthesized immunogen peptide.
Immunofluorescence Anti-TNFA: Cat. No. SAB4502982: Immunofluorescence of HepG2 cells using Anti-TNFA antibody. Cells on the left were treated with the Anti-TNFA antibody. Cells on the right (negative control) were treated with both Anti-TNFA antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 25 kDa |
| NCBI accession no. | P01375   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | mouse, human, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:10000 |
| immunofluorescence: 1:100-1:500 | |
| immunohistochemistry: 1:50-1:100 | |
| UniProt accession no. | P01375 |
| Biochem/physiol Actions: | The TNFA (tumor necrosis factor α) gene is significantly associated with the regulation of innate and adaptive immune responses. Polymorphism of TNFA gene is known to cause acute motor axonal neuropathy. The cytokine release and gene action is associated with epithelial cells, myeloid cells, endothelial, as well as tumor cells. Tumor cells are known to release TNFA, specifically in response to chemotherapy |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Anti-TNFA Antibody detects endogenous levels of total TNFA protein. |
| General description: | The TNFA (tumor necrosis factor α) gene is mapped to human chromosome 6p21.33 and codes for a pro-inflammatory cytokine. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human TNFA. Immunogen Range: 141-190 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |