Anti-p53 antibody produced in rabbit
SIGMA/SAB4503021 - affinity isolated antibody
Synonym: Antigen NY-CO-13; Cellular tumor antigen p53; Phosphoprotein p53; TP53; tumor protein p53
Product Type: Chemical
Immunohistochemistry Anti-p53: Cat. No. SAB4503021: Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using Anti-p53 antibody. Cells on the left were treated with the Anti-p53 antibody. Cells on the right (negative control) were treated with both Anti-p53 antibody and the synthesized immunogen peptide.
Immunofluorescence Anti-p53: Cat. No. SAB4503021: Immunofluorescence of HeLa cells using Anti-p53 antibody. Cells on the left were treated with the Anti-p53 antibody. Cells on the right (negative control) were treated with both Anti-p53 antibody and the synthesized immunogen peptide.
Western Blotting Anti-p53: Cat. No. SAB4503021: Immunoblotting analysis of extracts from COS7 cells, treated with TSA 400nM 24h, using Anti-p53 antibody. The lane on the left was treated with the Anti-p53 antibody. The lane on the right (negative control) was treated with both Anti-p53 antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 43 kDa |
| NCBI accession no. | P04637   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human, rat, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:20000 |
| immunofluorescence: 1:100-1:500 | |
| immunohistochemistry: 1:50-1:100 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P04637 |
| Biochem/physiol Actions: | TP53 gene is involved in the regulation of cell cycle, senescence and apoptosis upon oncogene activation, stress and DNA damage. Therefore, the gene has anti-tumor activity initiation and prevents tumor progression. Mutation in TP53 is known to be the major cause of almost half of all human malignancy. Breast cancer is one such condition in which, the disease development is severe due to mutation in TP53 gene. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Anti-p53 Antibody detects endogenous levels of total p53 protein. |
| General description: | TP53 gene is mapped to human chromosome 17p13.1. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human p53. Immunogen Range: 344-393 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |