Anti-C1S (heavy chain, Cleaved-Arg437) antibody produced in rabbit
SIGMA/SAB4503023 - affinity isolated antibody
Synonym: C1 esterase; C1S; Complement C1s subcomponent; Complement component 1 subcomponent s
Product Type: Chemical
Western Blotting Anti-C1S (heavy chain, Cleaved-Arg437): Cat. No. SAB4503023: Immunoblotting analysis of extracts from 293/HeLa cells, treated with etoposide 25uM 1h, using Anti-C1S (heavy chain, Cleaved-Arg437) antibody. The lane on the left was treated with the Anti-C1S (heavy chain, Cleaved-Arg437) antibody. The lane on the right (negative control) was treated with both Anti-C1S (heavy chain, Cleaved-Arg437) antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 47 kDa |
| antigen 76 kDa | |
| NCBI accession no. | P09871   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | proteolytically cleaved (Arg437) |
| technique(s) | ELISA: 1:20000 |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P09871 |
| Biochem/physiol Actions: | Activation of C1s (complement C1s) along with C1r triggers complement cascade. C1s is responsible for the cleavage of complement component C4 and C2, which is necessary for complete activation of complement system. Studies determined that C1s specifically acts on Val or Leu at P(3) and Ala or Gly residues at P(2) positions. The C1S gene is known to be associated lupus nephritis, with low gene expression observed in mice. |
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| General description: | Anti-C1S (heavy chain, Cleaved-Arg437 Antibody detects endogenous levels of fragment of activated C1S (heavy chain, Cleaved-Arg437protein. |
| General description: | The C1S (complement C1s) gene is mapped to human chromosome 12p13 and codes for a subcomponent of C1 complex of the classical pathway. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human C1S. Immunogen Range: 388-437 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |