Anti-Ubiquitin antibody produced in rabbit
SIGMA/SAB4503053 - affinity isolated antibody
Synonym: RPS27A; UBA52; UBA80; UBCEP1; Ubiquitin
Product Type: Chemical
Immunohistochemistry Anti-Ubiquitin: Cat. No. SAB4503053: Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Anti-Ubiquitin antibody. Cells on the left were treated with the Anti-Ubiquitin antibody. Cells on the right (negative control) were treated with both Anti-Ubiquitin antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 8 kDa |
| NCBI accession no. | P62988   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | mouse, rat, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:20000 |
| immunohistochemistry: 1:50-1:100 | |
| UniProt accession no. | P62987 |
| Application: | Anti-Ubiquitin antibody produced in rabbit has been used in following assays: • Western blot analysis. • Immunofluorescence-prox • Immunofluorescence. |
| Biochem/physiol Actions: | Ribosomal protein S27a (RPS27a) is involved in ribosome biogenesis and post-translational modifications of proteins. The encoded protein enhances cell proliferation, controls cell cycle progression, and inhibits apoptosis of leukemia cells. Overexpression of RPS27A or ubiquitin results in advanced-phase chronic myeloid leukemia (CML) and acute leukemia (AL). Hence drugs targeting RPS27a combining with tyrosine kinase inhibitor (TKI) might be a potential treatment for TKI resistant CML patients. Elevated expression of ubiquitin carboxyl extension protein 1 (UBCEP-1), which is a component of ubiquitin pathway, promotes prostate carcinoma development, via increasing the degradation of proteins involved in growth inhibition or apoptosis. RPS27a might act as a unique stress sensor in cells, which results in increased p53 activity leading to inhibition of cell cycle progression. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Anti-Ubiquitin Antibody detects endogenous levels of total Ubiquitin protein. |
| General description: | Ribosomal protein S27a (RPS27A), also known as ubiquitin carboxyl extension protein 1 (UBCEP-1), is a ribosomal protein, which is naturally synthesized as an ubiquitin (Ub) C-terminal extension protein. The protein is encoded by RPS27A gene mapped to human chromosome 2p16. RPS27 is a 9.8kDa protein characterized with one zinc finger domain in its monomeric state. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human Ubiquitin. Immunogen Range: 40-89 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |