Anti-Vinculin antibody produced in rabbit
SIGMA/SAB4503069 - affinity isolated antibody
Synonym: Metavinculin; VCL; VINC
MDL Number: MFCD00162905
Product Type: Chemical
Immunohistochemistry Anti-Vinculin: Cat. No. SAB4503069: Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Anti-Vinculin antibody. Cells on the left were treated with the Anti-Vinculin antibody. Cells on the right (negative control) were treated with both Anti-Vinculin antibody and the synthesized immunogen peptide.
Immunofluorescence Anti-Vinculin: Cat. No. SAB4503069: Immunofluorescence of A549 cells using Anti-Vinculin antibody. Cells on the left were treated with the Anti-Vinculin antibody. Cells on the right (negative control) were treated with both Anti-Vinculin antibody and the synthesized immunogen peptide.
Western Blotting Anti-Vinculin: Cat. No. SAB4503069: Immunoblotting analysis of extracts from HeLa cells, treated with forskolin 40nM 30′, using Anti-Vinculin antibody. The lane on the left was treated with the Anti-Vinculin antibody. The lane on the right (negative control) was treated with both Anti-Vinculin antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 123 kDa |
| NCBI accession no. | P18206   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | mouse, human, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:10000 |
| immunofluorescence: 1:100-1:500 | |
| immunohistochemistry: 1:50-1:100 | |
| western blot: 1:500-1:1000 |
| Biochem/physiol Actions: | Vinculin (VCL) is a cytoskeletal protein that supports cell-to-cell and cell to matrix junctions. It is also essentially involved in normal embryonic development and cardiac function, due to its role in controlling adhesion and motility, as well as cell spreading. In mature focal adhesions (FAs), VCL acts as an important constituent of the “molecular clutch” that induces the transmission of force from cytoplasmic F-actin to membrane-bound integrins. Aberration or mutation of VCL leads to cardiomyopathy, which is associated with hypertension. Experimental studies reveal that, VCL might be used as a biomarker of aging or heart failure. Vinculin associates with FAs and assists in several cellular functions, such as stabilizing and strengthening FAs and promoting their maturation, extracellular matrix (ECM) mechanosensing, regulating actin cytoskeletal dynamics and signaling pathways involved in apoptosis. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Vinculin (VCL) is a ubiquitously expressed cytoskeletal protein, encoded by the gene mapped to human chromosome 10q11.2-qter. VCL is a vital element of adherens type cell junctions. Vinculin structure contains a globular head connected to a tail domain by a short proline-rich sequence, and an intramolecular interaction between the head and tail hides the various ligand-binding sites in the protein. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human Vinculin. Immunogen Range: 786-835 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |