Anti-Vimentin antibody produced in rabbit
SIGMA/SAB4503081 - affinity isolated antibody
Synonym: VIME; vimentin
Product Type: Chemical
Western Blotting Anti-Vimentin: Cat. No. SAB4503081: Immunoblotting analysis of extracts from 293 cells, treated with EGF 200ng/ml 30′, using Anti-Vimentin antibody. The lane on the left was treated with the Anti-Vimentin antibody. The lane on the right (negative control) was treated with both Anti-Vimentin antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 53 kDa |
| NCBI accession no. | P08670   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human, mouse, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:10000 |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P08670 |
| Biochem/physiol Actions: | Vimentin is known to promote epithelial to mesenchymal transition and contributes to metastasis in cancer such as ovarian cancer. Vimentin preserves cellular structure and tissue integrity. Vimentin is also known to bring about TNF-α (tumor necrosis factor-α) induced cell apoptosis. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Anti-Vimentin Antibody detects endogenous levels of total Vimentin protein. |
| General description: | The VIM (vimentin) gene codes for an intermediate filament protein, that is expressed throughout mesenchymal cells. VIM gene is mapped to human chromosome 10p13. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human Vimentin. Immunogen Range: 56-105 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |