Anti-CA3 antibody produced in rabbit
SIGMA/SAB4503119 - affinity isolated antibody
Synonym: CA-III; Carbonate dehydratase III; Carbonic anhydrase 3; Carbonic anhydrase III
Product Type: Chemical
Western Blotting Anti-CA3: Cat. No. SAB4503119: Immunoblotting analysis of extracts from HuvEc cells, using Anti-CA3 antibody. The lane on the left was treated with the Anti-CA3 antibody. The lane on the right (negative control) was treated with both Anti-CA3 antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 29 kDa |
| NCBI accession no. | P07451   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | mouse, rat, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:20000 |
| western blot: 1:500-1:1000 |
| Application: | Anti-CA3 antibody produced in rabbit is suitable for indirect ELISA and western blot applications. |
| Biochem/physiol Actions: | Carbonic anhydrase III (CA3) may act as a new biomarker of sarcopenia. Higher CA levels indicate an increased demand of CO2 removal in senescent muscle and the age-related fibre type shifts to slower-contracting muscles during human aging. CA3 is found to be associated with rheumatoid arthritis. It is specifically insufficient in the skeletal muscle of patients suffering from Myasthenia gravis (MG) (an autoimmune disease mainly mediated by antibodies against acetylcholine receptor). CA3 possesses low carbon dioxide hydratase activity, resistance to the CA inhibitor acetazolamide unlike the other members of the CA family. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Anti-CA3 Antibody detects endogenous levels of total CA3 protein. |
| General description: | Carbonic anhydrase III (CA3) is a member of a multi-gene CA family that encodes carbonic anhydrase isozymes namely Car3 and CAIII. The expression of isozymes has been shown to abundantly increase during muscle aging and is relatively muscle specific. It exhibits a fibre type-specific expression pattern. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human CA3. Immunogen Range: 141-190 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |