Anti-Calnexin, C-Terminal antibody produced in rabbit
SIGMA/SAB4503258 - affinity isolated antibody
Synonym: CALX; CANX; IP90; Major histocompatibility complex class I
MDL Number: MFCD01094399
Product Type: Chemical
Immunohistochemistry Calnexin (Ab-583) Antibody: Cat. No. SAB4503258: Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using Calnexin (Ab-583) Antibody. The picture on the right is treated with the synthesized peptide.
Immunofluorescence Anti-Calnexin: Cat. No. SAB4503258: Immunofluorescence of HeLa cells using Anti-Calnexin antibody. Cells on the left were treated with the Anti-Calnexin antibody. Cells on the right (negative control) were treated with both Anti-Calnexin antibody and the synthesized immunogen peptide.
Western Blotting Anti-Calnexin: Cat. No. SAB4503258: Immunoblotting analysis of extracts from HeLa cells, treated with EGF 200ng/ml 30′, using Anti-Calnexin antibody. The lane on the left was treated with the Anti-Calnexin antibody. The lane on the right (negative control) was treated with both Anti-Calnexin antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 67 kDa |
| NCBI accession no. | P27824   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | mouse, rat, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:1000 |
| immunofluorescence: 1:100-1:500 | |
| immunohistochemistry: 1:50-1:100 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P27824 |
| Biochem/physiol Actions: | CANX (calnexin) participates in protein folding and quality control. It is responsible for glycan-independent interaction and retention of mutated Gas3/PMP22 (growth arrest-specific protein 3/peripheral myelin protein 22) proteins involved in Charcot-Marie-Tooth 1A and Dejerine Sottas syndrome. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Anti-Calnexin Antibody detects endogenous levels of total Calnexin protein. |
| General description: | CANX (calnexin) is an endoplasmic reticulum (ER) chaperone. It has a molecular weight of 67kDa. It is a type I transmembrane protein and is located on human chromosome 5q35. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human Calnexin. Immunogen Range: 543-592 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |