Anti-CASP-1 (Cleaved-Asp210) antibody produced in rabbit
SIGMA/SAB4503272 - affinity isolated antibody
Synonym: ICE; IL-1 β; IL-1 β converting enzyme; IL-1BC; caspase-1
Product Type: Chemical
Immunohistochemistry IL-1 beta (Cleaved-Asp210) Antibody: Cat. No. SAB4503272: Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using IL-1 beta (Cleaved-Asp210) Antibody. The picture on the right is treated with the synthesized peptide.
Western Blotting Anti-IL-1 β (Cleaved-Asp210): Cat. No. SAB4503272: Immunoblotting analysis of extracts from NIH-3T3 cells, treated with Etoposide 25uM 60′, using Anti-IL-1 β (Cleaved-Asp210) antibody. The lane on the left was treated with the Anti-IL-1 β (Cleaved-Asp210) antibody. The lane on the right (negative control) was treated with both Anti-IL-1 β (Cleaved-Asp210) antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 25 kDa |
| antigen 45 kDa | |
| NCBI accession no. | P29466   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | proteolytically cleaved (Asp210) |
| technique(s) | ELISA: 1:10000 |
| immunohistochemistry: 1:50-1:100 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P29466 |
| Biochem/physiol Actions: | Caspase-1 can transform pro-inflammatory cytokines, interleukin-1β (IL-1β) and interleukin-18 (IL-18) into their active forms. It participates in pyroptosis. It is overexpressed in the aorta of coronary atherosclerosis patients. CASP-1 helps in host cell survival by inducing membrane biogenesis to restore the impairment caused by pore-forming toxins. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Anti-CASP-1 (Cleaved-Asp210) Antibody detects endogenous levels of fragment of activated CASP-1 (Cleaved-Asp210) protein. |
| General description: | Caspase-1 is also known as interleukin-1β converting enzyme (ICE). It is located on human chromosome 11q22.2. It has 10 exons, an N terminal CARD (caspase activation and recruitment domain), a large P20 subunit and a small P10 subunit. CASP-1 is present in leukocytes, monocytes and epithelial cells. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human IL-1 beta. Immunogen Range: 161-210 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |