Anti-DARPP-32 antibody produced in rabbit
SIGMA/SAB4503329 - affinity isolated antibody
Synonym: Dopamine- and cAMP-regulated neuronal phosphoprotein; IPPD; Neuronal phosphoprotein DARPP-32; PPP1R1B
MDL Number: MFCD03454936
Product Type: Chemical
Immunohistochemistry Anti-DARPP-32: Cat. No. SAB4503329: Immunohistochemistry analysis of paraffin-embedded human brain tissue using Anti-DARPP-32 antibody. Cells on the left were treated with the Anti-DARPP-32 antibody. Cells on the right (negative control) were treated with both Anti-DARPP-32 antibody and the synthesized immunogen peptide.
Western Blotting Anti-DARPP-32: Cat. No. SAB4503329: Immunoblotting analysis of extracts from 293 cells, treated with EGF 200ng/ml 30′, using Anti-DARPP-32 antibody. The lane on the left was treated with the Anti-DARPP-32 antibody. The lane on the right (negative control) was treated with both Anti-DARPP-32 antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 22 kDa |
| NCBI accession no. | Q9UD71   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | mouse, rat, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:40000 |
| immunohistochemistry: 1:50-1:100 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | Q9UD71 |
| Biochem/physiol Actions: | DARPP-32 (dopamine and cAMP-regulated neuronal phosphoprotein 2) is a dopaminergic gene that influences dopamine receptor binding potential in the corpus striatum. The gene also regulates the availability of dopamine neurotransmitter, which is important for cognitive capacities. Therefore, variation in this gene affects category learning performance. (t)-DARPP takes part in the process of tumorigenesis by stimulating chemokine receptor 4 and also promoting cell proliferation. Elevated expression of the DARPP-32 gene in dorsolateral prefrontal neocortex and hippocampus is correlated with the development of schizophrenia and cognitive disorders. The encoded protein is a phosphorylation-dependent inhibitor. DARPP-32 is associated with the pathogenesis of gastric cancer and breast cancer with its anti-apoptotic property, and is found to promote angiogenesis. |
| Biochem/physiol Actions: | PPP1R1B (protein phosphatase 1 regulatory inhibitor subunit 1B)/DARPP32 (dopamine- and cAMP-regulated phosphoprotein of molecular weight 32 kDa) is considered as an inhibitor of phosphate-1 (PP-1) protein. It is associated with working memory and emotion processing. |
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| General description: | Anti-DARPP-32 Antibody detects endogenous levels of total DARPP-32 protein. |
| General description: | PPP1R1B (protein phosphatase 1 regulatory inhibitor subunit 1B)/DARPP32 (dopamine- and cAMP-regulated phosphoprotein of molecular weight 32 kDa) encodes two transcripts which are further translated into two DARPP-32 protein isoforms, full length (fl)-DARPP-32 and truncated (t)-DARPP. It is located on human chromosome 17q12. |
| General description: | The DARPP-32 (dopamine and cAMP-regulated neuronal phosphoprotein 2) gene is mapped to human chromosome 17q12. The gene is expressed predominantly in spiny neurons of the neostriatum and specifically localized in caudate, putamen, nucleus accumbens, cerebral and cerebellar cortex. The gene encodes two isoforms, full length (fl)-DARPP-32 and truncated (t)-DARPP. The lack of the first 36 residues at the N-terminal in t- DARPP marks the difference between the two isoforms. Anti-DARPP-32 Antibody detects endogenous levels of total DARPP-32 protein. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human DARPP-32. Immunogen Range: 41-90 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |