Anti-GPR174 antibody produced in rabbit
SIGMA/SAB4503376 - affinity isolated antibody
Synonym: FKSG79; GPR174; Probable G-protein coupled receptor 174
Product Type: Chemical
Immunofluorescence Anti-GPR174: Cat. No. SAB4503376: Immunofluorescence of LOVO cells using Anti-GPR174 antibody. Cells on the left were treated with the Anti-GPR174 antibody. Cells on the right (negative control) were treated with both Anti-GPR174 antibody and the synthesized immunogen peptide.
Western Blotting Anti-GPR174: Cat. No. SAB4503376: Immunoblotting analysis of extracts from K562 cells, using Anti-GPR174 antibody. The lane on the left was treated with the Anti-GPR174 antibody. The lane on the right (negative control) was treated with both Anti-GPR174 antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 38 kDa |
| NCBI accession no. | Q9BXC1   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:40000 |
| immunofluorescence: 1:100-1:500 | |
| western blot: 1:500-1:1000 |
| Biochem/physiol Actions: | G protein-coupled receptor 174 (GPR174) plays a vital role in inducing signal transduction in immune cells. GPR174 upon lysophosphatidylserine (LysoPS) binding, enhances the production of intracellular cyclic adenosine monophosphate (cAMP) in a dose dependent manner. Mutation in GPR174 gene can be a risk factor for Graves’ disease (GD) and autoimmune addison’s disease (AAD). Upregulation of intracellular cAMP by GPR174 leads to the inhibition of immune cell proliferation. GPR174, as a LysoPS receptor, might play a crucial role in the central nervous system. Alteration in the expression of the gene results in vasovagal syncope (VVS). Overexpression of GPR174 is observed in subcutaneous metastases. Lysophosphatidylserine (LysoPS) secreted by the immune system in vivo, acts as a ligand for GPR174. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | G protein-coupled receptor 174 (GPR174) is a 333 amino acid protein encoded by the gene with one exon mapped to human chromosome Xq21.1. GPR174 is a member of G protein–coupled receptor 1(GPCR1) / rhodopsin-like family. The encoded protein consists of seven α -helical transmembrane domains. The protein is predominantly expressed in immune related tissues, and it is expressed at low levels in thyroid. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human GPR174. Immunogen Range: 131-180 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |