Anti-HAT antibody produced in rabbit
SIGMA/SAB4503405 - affinity isolated antibody
Synonym: KAT1; histone acetyltransferase 1; histone acetyltransferase type B catalytic
Product Type: Chemical
Western Blotting Anti-HAT: Cat. No. SAB4503405: Immunoblotting analysis of extracts from K562 cells, using Anti-HAT antibody. The lane on the left was treated with the Anti-HAT antibody. The lane on the right (negative control) was treated with both Anti-HAT antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 49 kDa |
| NCBI accession no. | O14929   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | mouse, rat, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:5000 |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | O14929 |
| Application: | Anti-HAT antibody produced in rabbit has been used in western blotting (1:500) and immunofluorescence (1:250). |
| Biochem/physiol Actions: | Histone acetyltransferase 1 (HAT1) is involved in the acetylation of lysine 5- and 12- residues at the N-terminal of the newly synthesized histone H4. This enzyme is also involved in the regulation of DNA repair in the nucleus. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Histone acetyltransferase 1 (HAT1) is a type B histone acetyltransferase localized in the cytosol and the nucleus. The HAT1 gene is mapped on the human chromosome at 2q31.1. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human HAT. Immunogen Range: 331-380 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |