Anti-Actinin α antibody produced in rabbit
SIGMA/SAB4503474 - affinity isolated antibody
Synonym: α-actinin skeletal muscle isoform 2; α-actinin-2; α-actinin-3; ACTN2; F-actin cross-linking protein
Product Type: Chemical
Immunohistochemistry Anti-Actinin α: Cat. No. SAB4503474: Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue using Anti-Actinin α antibody. Cells on the left were treated with the Anti-Actinin α antibody. Cells on the right (negative control) were treated with both Anti-Actinin α antibody and the synthesized immunogen peptide.
Immunofluorescence Anti-Actinin α: Cat. No. SAB4503474: Immunofluorescence of HeLa cells using Anti-Actinin α antibody. Cells on the left were treated with the Anti-Actinin α antibody. Cells on the right (negative control) were treated with both Anti-Actinin α antibody and the synthesized immunogen peptide.
Western Blotting Anti-Actinin α: Cat. No. SAB4503474: Immunoblotting analysis of extracts from HepG2/HeLa/HuvEc cells, using Anti-Actinin α antibody. The lane on the left was treated with the Anti-Actinin α antibody. The lane on the right (negative control) was treated with both Anti-Actinin α antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 103 kDa |
| NCBI accession no. | P35609   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human, rat, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:20000 |
| immunofluorescence: 1:100-1:500 | |
| immunohistochemistry: 1:50-1:100 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P35609 |
| Biochem/physiol Actions: | Mutation in the α-actinin-2 (ACTN2) gene leads to hypertrophic cardiomyopathy. It is essential in cross-linking actin and titin filaments in the Z-disc. ACTN2 is associated with a number of cytoskeletal and signaling molecules and is involved in the structural and modulatory roles in the organization of the cytoskeleton and muscle contraction. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Anti-Actinin α Antibody detects endogenous levels of total Actinin α protein. α-actinin-2 (ACTN2) is a myofibrillar protein that is expressed in cardiac muscle. It is located at the the Z disk. The ACTN2 gene is mapped to human chromosome 1q43. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human Actinin alpha-2/3. Immunogen Range: 31-80 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |