Anti-Cyclin A antibody produced in rabbit
SIGMA/SAB4503499 - affinity isolated antibody
Synonym: Cyclin A1; cyclin A1
MDL Number: MFCD00243105
Product Type: Chemical
Immunohistochemistry Anti-Cyclin A: Cat. No. SAB4503499: Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using Anti-Cyclin A antibody. Cells on the left were treated with the Anti-Cyclin A antibody. Cells on the right (negative control) were treated with both Anti-Cyclin A antibody and the synthesized immunogen peptide.
Immunofluorescence Anti-Cyclin A: Cat. No. SAB4503499: Immunofluorescence of COS7 cells using Anti-Cyclin A antibody. Cells on the left were treated with the Anti-Cyclin A antibody. Cells on the right (negative control) were treated with both Anti-Cyclin A antibody and the synthesized immunogen peptide.
Western Blotting Anti-Cyclin A: Cat. No. SAB4503499: Immunoblotting analysis of extracts from COS7 cells, using Anti-Cyclin A antibody. The lane on the left was treated with the Anti-Cyclin A antibody. The lane on the right (negative control) was treated with both Anti-Cyclin A antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 52 kDa |
| NCBI accession no. | P78396   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | rat, human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:10000 |
| immunofluorescence: 1:100-1:500 | |
| immunohistochemistry: 1:50-1:100 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P78396 |
| Application: | Anti-Cyclin A antibody has been used in western blotting. |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Western Blotting (1 paper) |
| Biochem/physiol Actions: | CCNA1 (cyclin A1) controls cell apoptosis. Cyclin A2 is required for DNA replication and proliferation in somatic cells. CCNA1 plays an important role in head and neck carcinoma and cervical and nasopharyngeal cancers. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Anti-Cyclin A Antibody detects endogenous levels of total Cyclin A protein. |
| General description: | CCNA1 (cyclin A1) is a member of the highly conserved cyclin family. There are two A-type cyclins, namely cyclin A1 and cyclin A2. Cyclin A1 is expressed at high levels in testis and expressed at low levels in most other tissues. This gene is mapped to human chromosome 13q13. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human Cyclin A. Immunogen Range: 221-270 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |