Anti-Cyclin B1 antibody produced in rabbit
SIGMA/SAB4503501 - affinity isolated antibody
Synonym: CCN-2; CCNB; CCNB1; CCNB1-RS1; CYCB
MDL Number: MFCD00243104
Product Type: Chemical
Immunohistochemistry Anti-Cyclin B1: Cat. No. SAB4503501: Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Anti-Cyclin B1 antibody. Cells on the left were treated with the Anti-Cyclin B1 antibody. Cells on the right (negative control) were treated with both Anti-Cyclin B1 antibody and the synthesized immunogen peptide.
Immunofluorescence Anti-Cyclin B1: Cat. No. SAB4503501: Immunofluorescence of HeLa cells using Anti-Cyclin B1 antibody. Cells on the left were treated with the Anti-Cyclin B1 antibody. Cells on the right (negative control) were treated with both Anti-Cyclin B1 antibody and the synthesized immunogen peptide.
Western Blotting Anti-Cyclin B1: Cat. No. SAB4503501: Immunoblotting analysis of extracts from K562 cells, treated with serum 10% 15′, using Anti-Cyclin B1 antibody. The lane on the left was treated with the Anti-Cyclin B1 antibody. The lane on the right (negative control) was treated with both Anti-Cyclin B1 antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 48 kDa |
| NCBI accession no. | P14635   |
| shipped in | wet ice |
| species reactivity | rat, human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:20000 |
| immunofluorescence: 1:100-1:500 | |
| immunohistochemistry: 1:50-1:100 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P14635 |
| Application: | Anti-Cyclin B1 antibody has been used in western-blot analysis. |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Western Blotting (1 paper) |
| Biochem/physiol Actions: | Cyclin B1-CDK1 (cyclin-dependent kinase 1) helps to induce chromosome condensation, nuclear lamina resolution and mitotic spindle assembly. It also acts as a potential target for a cancer vaccine. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Anti-Cyclin B1 Antibody detects endogenous levels of total Cyclin B1 protein. |
| General description: | Cyclin B1 belongs to the cyclin family. It is a 48.337kDa protein. It is present in low level in testis, thymus, bone marrow and smooth muscle. It is mapped to human chromosome 5q13.2. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human Cyclin B1. Immunogen Range: 91-140 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |