Anti-PAR4 antibody produced in rabbit
SIGMA/SAB4503527 - affinity isolated antibody
Synonym: Coagulation factor II receptor-like 3; F2RL3; PAR-4; Proteinase-activated receptor 4; Thrombin receptor-like 3
MDL Number: MFCD02265744
Product Type: Chemical
Immunofluorescence Anti-PAR4: Cat. No. SAB4503527: Immunofluorescence of LOVO cells using Anti-PAR4 antibody. Cells on the left were treated with the Anti-PAR4 antibody. Cells on the right (negative control) were treated with both Anti-PAR4 antibody and the synthesized immunogen peptide.
Western Blotting Anti-PAR4: Cat. No. SAB4503527: Immunoblotting analysis of extracts from HepG2/Jurkat/HeLa cells, using Anti-PAR4 antibody. The lane on the left was treated with the Anti-PAR4 antibody. The lane on the right (negative control) was treated with both Anti-PAR4 antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 41 kDa |
| NCBI accession no. | Q96RI0   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | mouse, rat, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:10000 |
| immunofluorescence: 1:100-1:500 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | Q96RI0 |
| Application: | Anti-PAR4 antibody produced in rabbit has been used in immunoblotting (1:1000). |
| Biochem/physiol Actions: | Protease-activated receptor 4 (PAR4) protein is involved in vascular inflammation. It is necessary for a stable thrombus as it mediates persistent thrombin signaling in platelets. PAR4 aids pro-inflammatory actions by interacting with the bradykinin B2 receptor. It is linked to renal function and the development of chronic kidney disease (CKD). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Anti-PAR4 Antibody detects endogenous levels of total PAR4 protein. |
| General description: | F2R-like thrombin or trypsin receptor 3/coagulation factor 2 receptor-like 3 (F2RL3) gene codes for protease-activated receptor 4 (PAR4) protein. PAR4 belongs to the G protein-coupled receptors (GPCRs) family. It is expressed at high levels in the lungs, pancreas, thyroid, testis, and small intestine. PAR4 gene is located on human chromosome 19p12. Anti-PAR4 antibody detects endogenous levels of total PAR4 protein. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human PAR4. Immunogen Range: 29-78 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |