Anti-EXO1 antibody produced in rabbit
SIGMA/SAB4503568 - affinity isolated antibody
Synonym: EXO1; Exonuclease 1; Exonuclease I; hExo1; hExoI
Product Type: Chemical
Immunofluorescence Anti-EXO1: Cat. No. SAB4503568: Immunofluorescence of A549 cells using Anti-EXO1 antibody. Cells on the left were treated with the Anti-EXO1 antibody. Cells on the right (negative control) were treated with both Anti-EXO1 antibody and the synthesized immunogen peptide.
Western Blotting Anti-EXO1: Cat. No. SAB4503568: Immunoblotting analysis of extracts from A549 cells, using Anti-EXO1 antibody. The lane on the left was treated with the Anti-EXO1 antibody. The lane on the right (negative control) was treated with both Anti-EXO1 antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 94 kDa |
| NCBI accession no. | Q9UQ84   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:10000 |
| immunofluorescence: 1:100-1:500 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | Q9UQ84 |
| Application: | Anti-EXO1 antibody produced in rabbit has been used in immunoblotting. |
| Biochem/physiol Actions: | Exonuclease 1 (EXO1) N-domain mediates the DNA binding and the I domain is crucial for magnesium binding. It displays micro-mediated end-joining, 5′ to 3′ exonuclease activity and mediates homologous recombination and replication. EXO1 is more efficient on single-stranded DNA (ssDNA) than double-stranded DNA in exonucleolytic degradation. It also exhibits 5′ ssDNA-flap-specific endonuclease activity. EXO1 polymorphisms may be implicated in epithelial ovarian cancer (EOC). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Anti-EXO1 Antibody detects endogenous levels of total EXO1 protein. |
| General description: | Exonuclease 1 (EXO1), a member of RAD2 nuclease family, comprises an amino-terminal (N) domain, intermediate spacer region and an internal (I) domain with cysteine and glutamate residues. The Exo 1 gene is mapped to human chromosome 1q43. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human EXO1. Immunogen Range: 61-110 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |