Anti-DGKI antibody produced in rabbit
SIGMA/SAB4503570 - affinity isolated antibody
Synonym: DAG kinase ι; DGK-ι; DGKI; diacylglycerol kinase
Product Type: Chemical
Immunofluorescence Anti-DGKI: Cat. No. SAB4503570: Immunofluorescence of HeLa cells using Anti-DGKI antibody. Cells on the left were treated with the Anti-DGKI antibody. Cells on the right (negative control) were treated with both Anti-DGKI antibody and the synthesized immunogen peptide.
Western Blotting Anti-DGKI: Cat. No. SAB4503570: Immunoblotting analysis of extracts from 293 cells, using Anti-DGKI antibody. The lane on the left was treated with the Anti-DGKI antibody. The lane on the right (negative control) was treated with both Anti-DGKI antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 116 kDa |
| NCBI accession no. | O75912   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | rat, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:20000 |
| immunofluorescence: 1:100-1:500 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | O75912 |
| Application: | Anti-DGKI, C-Terminal antibody produced in rabbit is suitable for indirect immunofluorescence and western blot. |
| Biochem/physiol Actions: | DGKI (Diacylglycerol kinase iota) is mainly involved in the maintenance of intracellular concentration of diacylglycerol by phosphorylating other lipids in addition to DAG. DGKI has been predicted to be a potential gene for tumor suppression in colorectal cancers and mutation in DGKI has been linked to the melanoma. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Anti-DGKI Antibody detects endogenous levels of total DGKI protein. |
| General description: | DGKI (Diacylglycerol kinase iota) is a small, integral membrane protein belonging to the type IV diacylglycerol kinase subfamily. It is mapped on chromosome 7q32.3-q33. The members of the DGK family consist of a catalytic domain and at least two cysteine-rich regions. In type IV family members, it has four ankyrin repeats and a carboxy terminal PDZ binding domain. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human DGKI. Immunogen Range: 991-1040 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |