Anti-CD4 antibody produced in rabbit
SIGMA/SAB4503583 - affinity isolated antibody
Synonym: CD4; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4 precursor
Product Type: Chemical
Immunohistochemistry Anti-CD4: Cat. No. SAB4503583: Immunohistochemistry analysis of paraffin-embedded human brain tissue using Anti-CD4 antibody. Cells on the left were treated with the Anti-CD4 antibody. Cells on the right (negative control) were treated with both Anti-CD4 antibody and the synthesized immunogen peptide.
Immunofluorescence Anti-CD4: Cat. No. SAB4503583: Immunofluorescence of HeLa cells using Anti-CD4 antibody. Cells on the left were treated with the Anti-CD4 antibody. Cells on the right (negative control) were treated with both Anti-CD4 antibody and the synthesized immunogen peptide.
Western Blotting Anti-CD4: Cat. No. SAB4503583: Immunoblotting analysis of extracts from COLO205 cells, using Anti-CD4 antibody. The lane on the left was treated with the Anti-CD4 antibody. The lane on the right (negative control) was treated with both Anti-CD4 antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 51 kDa |
| NCBI accession no. | P01730   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:1000 |
| immunofluorescence: 1:100-1:500 | |
| immunohistochemistry: 1:50-1:100 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P01730 |
| Application: | Anti-CD4, C-Terminal antibody has been used in immunohistochemical staining. |
| Biochem/physiol Actions: | CD4 (CD4 molecule) helps in the activation of immunostimulatory T-cells. It serve as a primary receptor for HIV-1 entry.This molecule plays an important role on cell membrane mechanical characteristics and can be used in the development of cell biology and medicine. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Anti-CD4 Antibody detects endogenous levels of total CD4 protein. |
| General description: | CD4 (CD4 molecule) is present in the surface of specific leukocytes. CD4 contains four ecto-domains (D1-D4) of which D1, D2, and D4 contain disulfide bonds. It is mapped to human chromosome 12p13.31. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human CD4. Immunogen Range: 401-450 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |