Anti-EDG5 antibody produced in rabbit
SIGMA/SAB4503615 - affinity isolated antibody
Synonym: Endothelial differentiation G-protein coupled receptor 5; S1P receptor Edg-5; S1P2; Sphingosine 1-phosphate receptor 2; Sphingosine 1-phosphate receptor Edg-5
MDL Number: MFCD02686109
Product Type: Chemical
Western Blotting Anti-EDG5: Cat. No. SAB4503615: Immunoblotting analysis of extracts from HT-29 cells, using Anti-EDG5 antibody. The lane on the left was treated with the Anti-EDG5 antibody. The lane on the right (negative control) was treated with both Anti-EDG5 antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 38 kDa |
| NCBI accession no. | O95136   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | rat, human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:40000 |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | O95136 |
| Biochem/physiol Actions: | In NB (neuroblastoma), EDG5/ S1PR2 (sphingosine-1-phosphate receptor 2) helps in the expression of S1P-induced vascular endothelial growth factor. It promotes the Rho/Rho-kinase pathway. S1PR2 participates in S1P-induced platelet aggregation. S1PR2 is essential for normal auditory function as it is a part of the sphingosine-1-phosphate signaling (S1P) pathway. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
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| General description: | Anti-EDG5 Antibody detects endogenous levels of total EDG5 protein. EDG5/ S1PR2 (sphingosine-1-phosphate receptor 2), a pleiotropic lipid mediator is abundantly expressed in most of the tissues. S1PR2 codes for a 353 amino acid sphingosine-1-phosphate receptor 2 and is located on human chromosome 19p13.2. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human EDG5. Immunogen Range: 261-310 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |