Anti-CEP135 antibody produced in rabbit
SIGMA/SAB4503685 - affinity isolated antibody
Synonym: Centrosomal protein 4; Centrosomal protein of 135 kDa; Cep135
Product Type: Chemical
Western Blotting Anti-CEP135: Cat. No. SAB4503685: Immunoblotting analysis of extracts from COLO cells, using Anti-CEP135 antibody. The lane on the left was treated with the Anti-CEP135 antibody. The lane on the right (negative control) was treated with both Anti-CEP135 antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 133 kDa |
| NCBI accession no. | Q66GS9   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:40000 |
| western blot: 1:500-1:1000 |
| Application: | Anti-CEP135, C-Terminal antibody has been used in immunofluorescence staining. |
| Biochem/physiol Actions: | Mutation in Cep135 (centrosomal protein 135) result in primary microcephaly. It induces centriole assembly and stability. CEP135 helps to maintain the structure and organization of microtubules. This protein plays a major role in centriole biogenesis. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Anti-CEP135 Antibody detects endogenous levels of total CEP135 protein. |
| General description: | Cep135 (centrosomal protein 135) is a cartwheel protein. It has a coiled-coil domain and conserved regions near the N-terminus and the C-terminus, respectively. It is mapped to human chromosome 4q12. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human CEP135. Immunogen Range: 1081-1130 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |