Anti-MED24 antibody produced in rabbit
SIGMA/SAB4503717 - affinity isolated antibody
Synonym: Mediator complex subunit 24; Mediator of RNA polymerase II transcription subunit 24; Thyroid hormone receptor-associated protein 4; Trap100; hTRAP100
Product Type: Chemical
Immunofluorescence Anti-MED24: Cat. No. SAB4503717: Immunofluorescence of HuvEc cells using Anti-MED24 antibody. Cells on the left were treated with the Anti-MED24 antibody. Cells on the right (negative control) were treated with both Anti-MED24 antibody and the synthesized immunogen peptide.
Western Blotting Anti-MED24: Cat. No. SAB4503717: Immunoblotting analysis of extracts from 293 cells, using Anti-MED24 antibody. The lane on the left was treated with the Anti-MED24 antibody. The lane on the right (negative control) was treated with both Anti-MED24 antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 110 kDa |
| NCBI accession no. | O75448   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human, mouse, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:20000 |
| immunofluorescence: 1:100-1:500 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | O75448 |
| Application: | Anti-MED24, C-Terminal antibody produced in rabbit has been used in immunohistochemistry. |
| Biochem/physiol Actions: | Mediator complex subunit 24 (MED24)/ thyroid hormone receptor associated protein 100 (TRAP100), which is a subunit of TRAP coactivator complex, might promote TRAP-mediated enhancement of nuclear receptor - signaling pathways. The MED1/MED24 complex plays an essential role in the pubertal mammary gland development and growth of breast carcinoma cells via the rat sarcoma (RAS)-mitogen-activated protein kinase (MAPK) pathway. The MED1/MED24 complex is over-expressed in female breast cancer (FBC). In Drosophila, mutation of gene encoding MED24 inhibit salivary gland cell death, caspase activation, and the breakdown of nuclear lamins. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
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| General description: | Mediator complex subunit 24 (MED24), also known as thyroid hormone receptor associated protein 100 (TRAP100), is encoded by the gene mapped to human chromosome 17q21.1. The protein is ubiquitously expressed in human tissues, but at high level in skeletal muscle, heart, and placenta. TRAP100 is characterized with a notional zinc finger at N-terminal, a putative ATP/GTP binding site in the central region, and six dispersed LXXLL motifs. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human MED24. Immunogen Range: 801-850 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |