Anti-phospho-AMPK α (pThr172) antibody produced in rabbit
SIGMA/SAB4503754 - affinity isolated antibody
Synonym: Anti-AMPK; Anti-AMPK2; Anti-AMPKa2; Anti-PRKAA
Product Type: Chemical
Immunohistochemistry Anti-phoshpho-AMPK α (pThr172): Cat. No. SAB4503754: Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma.
Immunohistochemistry Anti-phoshpho-AMPK α (pThr172): Cat. No. SAB4503754: Immunohistochemistry analysis of paraffin-embedded Human kidney carcinoma.
Immunohistochemistry Anti-phoshpho-AMPK α (pThr172): Cat. No. SAB4503754: Immunohistochemistry analysis of paraffin-embedded Human liver carcinoma.
Immunohistochemistry Anti-phoshpho-AMPK α (pThr172): Cat. No. SAB4503754: Immunohistochemistry analysis of paraffin-embedded Human ovary carcinoma.
Immunohistochemistry Anti-phoshpho-AMPK α (pThr172): Cat. No. SAB4503754: Immunohistochemistry analysis of paraffin-embedded Human tonsil carcinoma.
Western Blotting Anti-phoshpho-AMPK α (pThr172): Cat. No. SAB4503754: Western blot analysis of extracts from COS-7 cells.
ELISA Anti-phoshpho-AMPK α (pThr172): Cat. No. SAB4503754: ELISA for Immunogen Phosphope(pTide (left) and Non-Phosphope(pTide (right).
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 62 kDa |
| NCBI accession no. | Q13131   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human, rat, mouse |
| storage temp. | −20°C |
| target post-translational modification | phosphorylation (pThr172) |
| technique(s) | ELISA: 1:40000 |
| immunohistochemistry: 1:50-1:100 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P54646 |
| Application: | Anti-phospho-AMPK α (pThr172) antibody produced in rabbit has been used in western blot analysis. |
| Application: | Anti-phospho-AMPK α (pThr172) antibody produced in rabbit has been used in western blotting. |
| Biochem/physiol Actions: | AMP-activated protein kinase is activated by the increased concentration of adenosine mono phosphate (AMP) during mitochondrial dysfunction. AMPK, or AMPK α1, is a vital constituent of the adaptive response to cardiomyocyte stress that occurs during ischemia. AMPK is involved in regulation of various cellular processes such as mitochondrial biogenesis, autophagy and inflammation, and immune responses. AMPK signaling pathway aids in maintaining energy balance in retinal neurons, therefore, it is considered as a potential therapeutic target to prevent or delay retinal degeneration. AMPK acts as a cellular energy sensor that controls glucose and lipid metabolism in response to change in nutrient and intracellular energy levels. C allele of protein kinase AMP-activated catalytic subunit α 1 (PRKAA1) rs13361707 is associated with susceptibility of gastric cancer (GCa). AMPKα2 plays an essential role in controlling postmortem glycolysis in skeletal muscle. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | AMP-activated protein kinase α (AMPKα) exists in two isoforms α1 and α2. The gene coding for AMPK α1 and AMPKα2 is mapped to human chromosome 5p11-p14 and 1p31 respectively. AMPKα is a 63kDa catalytic subunit of AMPK, involved in regulation of AMP levels. The AMP-activated protein kinase (AMPK) is a ubiquitously expressed cellular energy sensor. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human AMPK alpha around the phosphorylation site of Thr172. Immunogen Range: 140-189 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |