Anti-phospho-Smad1 (pSer465) antibody produced in rabbit
SIGMA/SAB4503779 - affinity isolated antibody
Product Type: Chemical
Immunohistochemistry Anti-phoshpho-Smad1 (pSer465): Cat. No. SAB4503779: Immunohistochemistry analysis of paraffin-embedded Human brain gliomas.
Immunohistochemistry Anti-phoshpho-Smad1 (pSer465): Cat. No. SAB4503779: Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma.
Immunohistochemistry Anti-phoshpho-Smad1 (pSer465): Cat. No. SAB4503779: Immunohistochemistry analysis of paraffin-embedded Human kidney carcinoma.
Immunohistochemistry Anti-phoshpho-Smad1 (pSer465): Cat. No. SAB4503779: Immunohistochemistry analysis of paraffin-embedded Human liver carcinoma.
Immunohistochemistry Anti-phoshpho-Smad1 (pSer465): Cat. No. SAB4503779: Immunohistochemistry analysis of paraffin-embedded Human Non-Hodgkin′s lymphoma.
Immunohistochemistry Anti-phoshpho-Smad1 (pSer465): Cat. No. SAB4503779: Immunohistochemistry analysis of paraffin-embedded Human stomach carcinoma.
Western Blotting Anti-phoshpho-Smad1 (pSer465): Cat. No. SAB4503779: Western blot analysis of extracts from HeLa cells treated with 10% serum for 5 minutes.
ELISA Anti-phoshpho-Smad1 (pSer465): Cat. No. SAB4503779: ELISA for Immunogen Phosphope(pTide (left) and Non-Phosphope(pTide (right).
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 52 kDa |
| NCBI accession no. | Q15797   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | mouse, rat, human |
| storage temp. | −20°C |
| target post-translational modification | phosphorylation (pSer465) |
| technique(s) | ELISA: 1:20000 |
| immunohistochemistry: 1:50-1:100 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | Q15797 |
| Biochem/physiol Actions: | SMAD (homologues of the Drosophila protein, mothers against decapentaplegic (Mad) and the Caenorhabditis elegans protein Sma) proteins have a major role in disease development and hence act as biologic prognostic factors in diseases. miR-345 represses the multiplication, invasion and migration of prostate cancer by downregulating Smad1. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Smad1 (SMAD family member 1) is a transcriptional activator, that is located on human chromosome 4q31. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human Smad1 around the phosphorylation site of Ser465. Immunogen Range: 416-465 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |