Anti-phospho-CDC25B (pSer323) antibody produced in rabbit
SIGMA/SAB4503810 - affinity isolated antibody
Product Type: Chemical
Immunohistochemistry Anti-phoshpho-CDC25B (pSer323): Cat. No. SAB4503810: Immunohistochemistry analysis of paraffin-embedded Human brain gliomas.
Immunohistochemistry Anti-phoshpho-CDC25B (pSer323): Cat. No. SAB4503810: Immunohistochemistry analysis of paraffin-embedded Human breast carcinoma.
Immunohistochemistry Anti-phoshpho-CDC25B (pSer323): Cat. No. SAB4503810: Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma.
Immunohistochemistry Anti-phoshpho-CDC25B (pSer323): Cat. No. SAB4503810: Immunohistochemistry analysis of paraffin-embedded Human kidney carcinoma.
Immunohistochemistry Anti-phoshpho-CDC25B (pSer323): Cat. No. SAB4503810: Immunohistochemistry analysis of paraffin-embedded Human liver carcinoma.
Immunohistochemistry Anti-phoshpho-CDC25B (pSer323): Cat. No. SAB4503810: Immunohistochemistry analysis of paraffin-embedded Human lung carcinoma.
Immunohistochemistry Anti-phoshpho-CDC25B (pSer323): Cat. No. SAB4503810: Immunohistochemistry analysis of paraffin-embedded Human Non-Hodgkin′s lymphoma.
Immunohistochemistry Anti-phoshpho-CDC25B (pSer323): Cat. No. SAB4503810: Immunohistochemistry analysis of paraffin-embedded Human prostate carcinoma.
Western Blotting Anti-phoshpho-CDC25B (pSer323): Cat. No. SAB4503810: Western blot analysis of extracts from NIH-3T3 cells treated with 125ng/mL PMA for 30 minutes.
ELISA Anti-phoshpho-CDC25B (pSer323): Cat. No. SAB4503810: ELISA for Immunogen Phosphope(pTide (left) and Non-Phosphope(pTide (right).
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 64 kDa |
| NCBI accession no. | P30305   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | mouse, rat, human |
| storage temp. | −20°C |
| target post-translational modification | phosphorylation (pSer323) |
| technique(s) | ELISA: 1:20000 |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P30305 |
| Biochem/physiol Actions: | The expression of CDC25B (cell division cycle 25B) can be considered as a prognostic marker in the pathogenesis of retinoblastoma. It helps to dephosphorylate CDK1 (cyclin-dependent kinase 1) and ERK1/2 (extracellular signal-regulated kinase 1/2). It controls the cell cycle. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | CDC25B (cell division cycle 25B), a short half-life protein belongs to the dual-specificity CDC25 phosphatases family. It is located on human chromosome 20p. The protein is upregulated in late G2 of the cell cycle. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human CDC25B around the phosphorylation site of Ser323. Immunogen Range: 289-338 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |