Anti-phospho-p130 Cas (pTyr410) antibody produced in rabbit
SIGMA/SAB4503824 - affinity isolated antibody
Product Type: Chemical
Immunohistochemistry Anti-phoshpho-p130 Cas (pTyr410): Cat. No. SAB4503824: Immunohistochemistry analysis of paraffin-embedded Human brain gliomas.
Immunohistochemistry Anti-phoshpho-p130 Cas (pTyr410): Cat. No. SAB4503824: Immunohistochemistry analysis of paraffin-embedded Human cervical carcinoma.
Immunohistochemistry Anti-phoshpho-p130 Cas (pTyr410): Cat. No. SAB4503824: Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma.
Immunohistochemistry Anti-phoshpho-p130 Cas (pTyr410): Cat. No. SAB4503824: Immunohistochemistry analysis of paraffin-embedded Human kidney carcinoma.
Immunohistochemistry Anti-phoshpho-p130 Cas (pTyr410): Cat. No. SAB4503824: Immunohistochemistry analysis of paraffin-embedded Human stomach carcinoma.
Western Blotting Anti-phoshpho-p130 Cas (pTyr410): Cat. No. SAB4503824: Western blot analysis of extracts from NIH-3T3 cells.
ELISA Anti-phoshpho-p130 Cas (pTyr410): Cat. No. SAB4503824: ELISA for Immunogen Phosphope(pTide (left) and Non-Phosphope(pTide (right).
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 93 kDa |
| NCBI accession no. | P56945   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | mouse, rat, human |
| storage temp. | −20°C |
| target post-translational modification | phosphorylation (pTyr410) |
| technique(s) | ELISA: 1:5000 |
| immunohistochemistry: 1:50-1:100 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P56945 |
| Biochem/physiol Actions: | Breast cancer antiestrogen resistance 1 (BCAR1), also known as p130 Cas, plays a vital role in various cellular processes such as migration, adhesion, cytoskeleton reorganization host-pathogen interaction, survival, proliferation, transformation, and possibly metastasis. It is associated with the growth factor receptor EGFR and the metastasis suppressor KAI1, in vitro. Overexpression of BCAR1 in localized prostate cancers (LPC) suggests that this protein has potential as a marker for both prognosis and treatment. It is also considered to be a potential marker for breast cancer. Neural precursor cell expressed developmentally down-regulated protein 9 (NEDD-9) and BCAR1, via SRC, promote removal of E-Cadherin from cell membrane, and trigger E-cadherin degradation. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Breast cancer antiestrogen resistance 1 (BCAR1), also known as p130 Cas, is encoded by the gene mapped to human chromosome 16q23. BCAR1 belongs to Cas (Crk-associated substrate) protein family. The protein is characterized with an amino-terminal Src homology 3 (SH3) domain, a Src-binding domain, a large substrate domain, and a helix-loop-helix domain. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human p130 Cas around the phosphorylation site of Tyr410. Immunogen Range: 376-425 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |