Anti-phospho-IKK-α (pSer176) /IKK-β (pSer177) antibody produced in rabbit
SIGMA/SAB4503882 - affinity isolated antibody
Product Type: Chemical
Immunohistochemistry Anti-phoshpho-IKK-α (pSer176)/IKK-beta (pSer177): Cat. No. SAB4503882: Immunohistochemistry analysis of paraffin-embedded Human brain gliomas.
Immunohistochemistry Anti-phoshpho-IKK-α (pSer176)/IKK-beta (pSer177): Cat. No. SAB4503882: Immunohistochemistry analysis of paraffin-embedded Human liver carcinoma.
Western Blotting Anti-phoshpho-IKK-α (pSer176)/IKK-beta (pSer177): Cat. No. SAB4503882: Western blot analysis of extracts from NIH-3T3 cells treated with 20 ng/mL TNF-α for 30 minutes.
ELISA Anti-phoshpho-IKK-α (pSer176)/IKK-beta (pSer177): Cat. No. SAB4503882: ELISA for Immunogen Phosphope(pTide (left) and Non-Phosphope(pTide (right).
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 84 kDa |
| NCBI accession no. | O14920   |
| O15111 |
|
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | rat, human, mouse |
| storage temp. | −20°C |
| target post-translational modification | phosphorylation (pSer176/pSer177) |
| technique(s) | ELISA: 1:1000 |
| immunohistochemistry: 1:50-1:100 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | O15111 |
| Biochem/physiol Actions: | IKK1/IKKα (I-κ-B kinase 1) modulates nuclear factor κ-B (NF-κB) signaling, that is required for lymphoid organogenesis and adaptive immunity. IκB kinase α (IKKα) plays a major role in human skin cancer. This gene also controls terminal differentiation and multiplication of keratinocytes. IKKβ acts as the master regulator of cell survival and the inflammatory response. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | CHUK (conserved helix-loop-helix ubiquitous kinase) is located on human chromosome 10q24. IKK1/IKK-α has three ordered protein domains, namely, a kinase domain (KD), a ubiquitin-like domain (ULD) and a scaffold dimerization domain (SDD). IKKB (inhibitor of nuclear factor κ B kinase subunit β) is mapped to human chromosome 8p11. IKBKB codes for IκB kinase 2 (IKK2/IKKβ). IKK1 and IKK2 has a canonical MAP kinase kinase (MAPKK) activation loop motif. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human IKK-alpha around the phosphorylation site of Ser177. Immunogen Range: 151-200 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |