Anti-phospho-Aurora Kinase (pThr288) antibody produced in rabbit
SIGMA/SAB4503898 - affinity isolated antibody
Product Type: Chemical
Western Blotting Anti-phoshpho-Aurora Kinase (pThr288): Cat. No. SAB4503898: Western blot analysis of extracts from HEK 293 cells treated with 20% serum for 5 minutes.
ELISA Anti-phoshpho-Aurora Kinase (pThr288): Cat. No. SAB4503898: ELISA for Immunogen Phosphope(pTide (left) and Non-Phosphope(pTide (right).
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 45 kDa |
| NCBI accession no. | O14965   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human, rat, mouse |
| storage temp. | −20°C |
| target post-translational modification | phosphorylation (pThr288) |
| technique(s) | ELISA: 1:1000 |
| immunohistochemistry: 1:50-1:100 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | O14965 |
| Application: | Anti-phospho-Aurora Kinase (pThr288) antibody produced in rabbit has been used in immunofluorescence staining. |
| Biochem/physiol Actions: | Aurora kinase A (AURKA) is involved in maintaining the stability of mitosis and chromosomes. During mitosis, AURKA participates in centrosome maturation control, mitotic entry, bipolar spindle formation, and segregation. AURKA also participates in chromosome alignment and cytokinesis. It controls several oncogenic signaling pathways. AURKA plays a major role in the self-renewal, reprogramming, and differentiation of stem cells. Knockdown of AURKA gene affects the maturation of the centrosome. Abnormality in the AURKA gene leads to aberrant cell division and aneuploidy, which in turn affects the stability of the genome and contributes to carcinogenesis. Overexpression of the AURKA gene is seen in gastrointestinal, pancreatic, and liver cancers. It can act as a candidate gene to predict colorectal adenocarcinoma. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Aurora kinase A (AURKA) belongs to the serine/threonine kinase family. It is localized to centrosomes. AURKA gene is located on human chromosome 20q13.2. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human Aurora Kinase around the phosphorylation site of Thr288. Immunogen Range: 256-305 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |