Anti-phospho-Akt (pTyr474) antibody produced in rabbit
SIGMA/SAB4504014 - affinity isolated antibody
Product Type: Chemical
Immunohistochemistry Anti-phoshpho-Akt (pTyr474): Cat. No. SAB4504014: Immunohistochemistry analysis of paraffin-embedded Human breast carcinoma.
Immunohistochemistry Anti-phoshpho-Akt (pTyr474): Cat. No. SAB4504014: Immunohistochemistry analysis of paraffin-embedded Human kidney carcinoma.
Immunohistochemistry Anti-phoshpho-Akt (pTyr474): Cat. No. SAB4504014: Immunohistochemistry analysis of paraffin-embedded Human Non-Hodgkin′s lymphoma.
Immunohistochemistry Anti-phoshpho-Akt (pTyr474): Cat. No. SAB4504014: Immunohistochemistry analysis of paraffin-embedded Human ovary carcinoma.
Immunohistochemistry Anti-phoshpho-Akt (pTyr474): Cat. No. SAB4504014: Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue.
Western Blotting Anti-phoshpho-Akt (pTyr474): Cat. No. SAB4504014: Western blot analysis of extracts from COS-7 cells treated with UV for 5 minutes.
ELISA Anti-phoshpho-Akt (pTyr474): Cat. No. SAB4504014: ELISA for Immunogen Phosphope(pTide (left) and Non-Phosphope(pTide (right).
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 55 kDa |
| NCBI accession no. | P31749   |
| shipped in | wet ice |
| species reactivity | human, mouse, rat |
| storage temp. | −20°C |
| target post-translational modification | phosphorylation (pTyr474) |
| technique(s) | ELISA: 1:5000 |
| immunohistochemistry: 1:50-1:100 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P31749 |
| Biochem/physiol Actions: | The protein encoded by AKT1 (AKT serine/threonine kinase 1) gene controls the survival of cells and anti-apoptotic actions that attack the pathogenesis of several cancers, hence it plays a crucial role in tumorigenesis. In mice, AKT1 is essential for normal development. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | The AKT1 (AKT serine/threonine kinase 1) gene is also called as v-akt murine thymoma viral oncogene homolog 1 and PKBα (protein kinase B). It is upregulated in liver, muscle and adipocytes. AKT1 gene is mapped to human chromosome 14q32. It is a member of AKT family that has an amino-terminal pleckstrin homology (PH) domain, a short α-helical linker and a carboxyl-terminal kinase domain. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human Akt around the phosphorylation site of Tyr474. Immunogen Range: 431-480 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |