Anti-phospho-ATP-Citrate Lyase (pSer454) antibody produced in rabbit
SIGMA/SAB4504020 - affinity isolated antibody
Product Type: Chemical
Immunohistochemistry Anti-phoshpho-ATP-Citrate Lyase (pSer454): Cat. No. SAB4504020: Immunohistochemistry analysis of paraffin-embedded Human breast carcinoma.
Immunohistochemistry Anti-phoshpho-ATP-Citrate Lyase (pSer454): Cat. No. SAB4504020: Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma.
Immunohistochemistry Anti-phoshpho-ATP-Citrate Lyase (pSer454): Cat. No. SAB4504020: Immunohistochemistry analysis of paraffin-embedded Human kidney carcinoma.
Immunohistochemistry Anti-phoshpho-ATP-Citrate Lyase (pSer454): Cat. No. SAB4504020: Immunohistochemistry analysis of paraffin-embedded Human ovary carcinoma.
Immunohistochemistry Anti-phoshpho-ATP-Citrate Lyase (pSer454): Cat. No. SAB4504020: Immunohistochemistry analysis of paraffin-embedded Human prostate carcinoma.
Immunohistochemistry Anti-phoshpho-ATP-Citrate Lyase (pSer454): Cat. No. SAB4504020: Immunohistochemistry analysis of paraffin-embedded Human stomach carcinoma.
Western Blotting Anti-phoshpho-ATP-Citrate Lyase (pSer454): Cat. No. SAB4504020: Western blot analysis of extracts from COS-7 cells treated with 50 nM Calyculin for 30 minutes.
ELISA Anti-phoshpho-ATP-Citrate Lyase (pSer454): Cat. No. SAB4504020: ELISA for Immunogen Phosphope(pTide (left) and Non-Phosphope(pTide (right).
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 120 kDa |
| NCBI accession no. | P53396   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | mouse, rat, human |
| storage temp. | −20°C |
| target post-translational modification | phosphorylation (pSer454) |
| technique(s) | ELISA: 1:1000 |
| immunohistochemistry: 1:50-1:100 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P53396 |
| Application: | Anti-phospho-ATP-Citrate Lyase (pSer454) antibody produced in rabbit has been used in immunohistochemistry. |
| Biochem/physiol Actions: | ACLY (ATP citrate lyase) is responsible for cleavage of mitochondria-derived citrate to generate oxaloacetic acid (OAA) and acetyl-CoA, which is required for lipid biogenesis. This enzyme couples energy metabolism with fatty acids and facilitates cell growth. In addition, ACLY is also essential for DNA-damage-induced histone acetylation. ACLY is upregulated in several types of cancers and is associated with cancer cell proliferation. Elevated expression of the gene leads to the development of breast cancer. Thus, ACLY -related inhibitors can be considered as a potential strategy for treating breast cancer. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | ATP citrate lyase (ACLY) is encoded by the gene mapped to human chromosome 17q21.2. It is localized in the cytoplasm and is widely expressed in mammalian tissues.. ACLY is a 440 KDa tetrameric protein containing four apparently identical subunits. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human ATP-Citrate Lyase around the phosphorylation site of Ser454. Immunogen Range: 420-469 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |