Anti-phospho-PKC-pan (pThr497) antibody produced in rabbit
SIGMA/SAB4504099 - affinity isolated antibody
Synonym: Anti-PKCL; Anti-PRKCL; Anti-nPKC-eta
Product Type: Chemical
Immunohistochemistry Anti-phoshpho-PKC-pan (pThr497): Cat. No. SAB4504099: Immunohistochemistry analysis of paraffin-embedded Human cervical carcinoma.
Immunohistochemistry Anti-phoshpho-PKC-pan (pThr497): Cat. No. SAB4504099: Immunohistochemistry analysis of paraffin-embedded Human cervical carcinoma.
Immunohistochemistry Anti-phoshpho-PKC-pan (pThr497): Cat. No. SAB4504099: Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma.
Immunohistochemistry Anti-phoshpho-PKC-pan (pThr497): Cat. No. SAB4504099: Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma.
Immunohistochemistry Anti-phoshpho-PKC-pan (pThr497): Cat. No. SAB4504099: Immunohistochemistry analysis of paraffin-embedded Human prostate carcinoma.
Western Blotting Anti-phoshpho-PKC-pan (pThr497): Cat. No. SAB4504099: Western blot analysis of extracts from NIH-3T3 cells treated with PMA 250ng/mL for 5 minutes.
ELISA Anti-phoshpho-PKC-pan (pThr497): Cat. No. SAB4504099: ELISA for Immunogen Phosphope(pTide (left) and Non-Phosphope(pTide (right).
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 76 kDa |
| NCBI accession no. | P05129   |
| P05771 |
|
| P17252 |
|
| P24723 |
|
| Q02156 |
|
| Q04759 |
|
| Q05513 |
|
| Q05655 |
|
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | rat, mouse, human |
| storage temp. | −20°C |
| target post-translational modification | phosphorylation (pThr497) |
| technique(s) | ELISA: 1:40000 |
| immunofluorescence: 1:100-1:500 | |
| immunohistochemistry: 1:50-1:100 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P24723 |
| Application: | Anti-phospho-PKC-pan (pThr497) antibody produced in rabbit has been used in western blot analysis (1:500). |
| Biochem/physiol Actions: | Protein kinase Cs (PKCs) are involved in the growth and function of the gastrointestinal tract and the immune response. PKCs regulates cyclins, cyclin-dependent kinases (cdks), cdk inhibitors, and cell division cycle 25 (cdc25) phosphatases. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Protein kinase C is a member of the cAMP-dependent, cGMP-dependent, and protein kinase C (AGC) superfamily of protein kinases. It has more than14 isoforms. A catalytic domain with an ATP binding site and a substrate-binding site is found in all PKC isoforms. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human PKC-pan around the phosphorylation site of Thr497. Immunogen Range: 623-672 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |